ArticlePediatric surgery international2003
Interleukin-6 changes tight junction permeability and intracellular phospholipid content in a human enterocyte cell culture model.
Article in Pediatric surgery international, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 48 citations in OpenAlex.
- Modulating the Gut-Muscle Axis: Increasing SCFA-Producing Gut Microbiota Commensals and Decreasing Endotoxin Production to Mitigate Cancer Cachexia.Microorganisms · 2025Review
- Exploring the therapeutic potential of cinnamoyl derivatives in attenuating inflammation in lipopolysaccharide-induced Caco-2 cells.Future medicinal chemistry · 2024Article
- The leaky gut and the gut microbiome in sepsis - targets in research and treatment.Clinical science (London, England : 1979) · 2023Article
- Article
- Opioid Use, Gut Dysbiosis, Inflammation, and the Nervous System.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2022Review
- Corosolic Acid Inhibits Secretory Phospholipase AJournal of inflammation research · 2022Article
- Fat of the Gut: Epithelial Phospholipids in Inflammatory Bowel Diseases.International journal of molecular sciences · 2021Review
- The JAK Inhibitor Tofacitinib Rescues Intestinal Barrier Defects Caused by Disrupted Epithelial-macrophage Interactions.Journal of Crohn's & colitis · 2021Article
- Tight Junctions as a Key for Pathogens Invasion in Intestinal Epithelial Cells.International journal of molecular sciences · 2021Review
- Ganglioside Alters Phospholipase Trafficking, Inhibits NF-κB Assembly, and Protects Tight Junction Integrity.Frontiers in nutrition · 2021Article
- Effects ofPharmaceuticals (Basel, Switzerland) · 2018Article
- Lactobacillus reuteri strains protect epithelial barrier integrity of IPEC-J2 monolayers from the detrimental effect of enterotoxigenic Escherichia coli.Physiological reports · 2018Article
- Article
- Interleukin-6 modulation of intestinal epithelial tight junction permeability is mediated by JNK pathway activation of claudin-2 gene.PloS one · 2014Article
- p38 MAPK mediates calcium oxalate crystal-induced tight junction disruption in distal renal tubular epithelial cells.Scientific reports · 2013Article
- Lipopolysaccharide-induced bacterial translocation is intestine site-specific and associates with intestinal mucosal inflammation.Inflammation · 2012Article
- Inflammatory mediators weaken the amniotic membrane barrier through disruption of tight junctions.The Journal of physiology · 2010Article
- Macrophages increase microparticle uptake by enterocyte-like Caco-2 cell monolayers.Journal of anatomy · 2010Article
- Mechanism of cytokine modulation of epithelial tight junction barrier.Frontiers in bioscience (Landmark edition) · 2009Review
- Oxidative stress-induced disruption of epithelial and endothelial tight junctions.Frontiers in bioscience : a journal and virtual library · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proinflammatory cytokines and secretory phospholipase A(2) (sPLA(2)) are elevated in patients with inflammatory bowel disease (IBD). We previously reported that the proinflammatory cytokine IL-6 increased the expression of sPLA(2) (a hydrolyzer of phosphatidylcholine) and decreased membrane integrity in an intestinal epithelial cell culture model. To determine the physiological effects of the IL-6 mediated increase in sPLA(2) on decreased epithelial layer integrity, we investigated alterations of intracellular/secretory phospholipid (PL) composition in a cell culture model. In addition, since other PLs may also mediate epithelial membrane activity, we investigated the effect of IL-6 on PL activity in a Caco-2 enterocyte culture model. Caco-2 cells were incubated for 72 h with IL-6 or media alone (control). Both media and cell lysate were analyzed for PL composition using thin-layer chromatography. The PL composition in the media did not show any differences between the two groups ( p>0.1). Total intracellular PL contents were also unchanged; however, IL-6 led to significant changes in PL composition including an increase in phosphatidylethanolamine (PE) and sphingomyelin (SM) and a decrease in phosphatidylcholine (PC) and lysophosphatidylcholine (LPC) ( p<0.05). Both PE and SM are known as inflammatory signaling factors involved in human IBD. Our study suggests that the decreased membrane integrity seen with IL-6 application may occur via intracellular PL alterations, rather than through the direct effects of sPLA(2).
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