ArticleApplied and environmental microbiology2003
In situ accessibility of Saccharomyces cerevisiae 26S rRNA to Cy3-labeled oligonucleotide probes comprising the D1 and D2 domains.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- In Vitro Study of Probiotic Properties and Safety Aspects ofMolecules (Basel, Switzerland) · 2026Article
- Pan-cancer analyses reveal cancer-type-specific fungal ecologies and bacteriome interactions.Cell · 2022Article
- An important step forward for the future development of an easy and fast procedure for identifying the most dangerous wine spoilage yeast, Dekkera bruxellensis, in wine environment.Microbial biotechnology · 2019Article
- Stress induced biofilm formation in Propionibacterium acidipropionici and use in propionic acid production.World journal of microbiology & biotechnology · 2019Article
- Considerations and consequences of allowing DNA sequence data as types of fungal taxa.IMA fungus · 2018Article
- Basidiomycete yeasts in the cortex of ascomycete macrolichens.Science (New York, N.Y.) · 2016Article
- Design and evaluation of peptide nucleic acid probes for specific identification of Candida albicans.Journal of clinical microbiology · 2015Article
- Fine structure of Tibetan kefir grains and their yeast distribution, diversity, and shift.PloS one · 2014Article
- Molecular techniques for pathogen identification and fungus detection in the environment.IMA fungus · 2011Article
- A Transcriptome-Targeting EcoChip for Assessing Functional Mycodiversity.Microarrays (Basel, Switzerland) · 2011Article
- Comparison of quantitative real time PCR with Sequencing and ribosomal RNA-FISH for the identification of fungi in formalin fixed, paraffin-embedded tissue specimens.BMC infectious diseases · 2011Article
- Direct and specific identification of Cryptococcus neoformans in biological samples using fluorescently labelled DNA probes.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2010Article
- Multiple simultaneous detection of Harmful Algal Blooms (HABs) through a high throughput bead array technology, with potential use in phytoplankton community analysis.Harmful algae · 2009Article
- In situ detection of freshwater fungi in an alpine stream by new taxon-specific fluorescence in situ hybridization probes.Applied and environmental microbiology · 2008Article
- Evaluation of sequence alignments and oligonucleotide probes with respect to three-dimensional structure of ribosomal RNA using ARB software package.BMC bioinformatics · 2006Article
- Use of a suspension array for rapid identification of the varieties and genotypes of the Cryptococcus neoformans species complex.Journal of clinical microbiology · 2005Article
- Graphical representation of ribosomal RNA probe accessibility data using ARB software package.BMC bioinformatics · 2005Article
- Is the in situ accessibility of the 16S rRNA of Escherichia coli for Cy3-labeled oligonucleotide probes predicted by a three-dimensional structure model of the 30S ribosomal subunit?Applied and environmental microbiology · 2003Article
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6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence in situ hybridization (FISH) has proven to be most useful for the identification of microorganisms. However, species-specific oligonucleotide probes often fail to give satisfactory results. Among the causes leading to low hybridization signals is the reduced accessibility of the targeted rRNA site to the oligonucleotide, mainly for structural reasons. In this study we used flow cytometry to determine whole-cell fluorescence intensities with a set of 32 Cy3-labeled oligonucleotide probes covering the full length of the D1 and D2 domains in the 26S rRNA of Saccharomyces cerevisiae PYCC 4455(T). The brightest signal was obtained with a probe complementary to positions 223 to 240. Almost half of the probes conferred a fluorescence intensity above 60% of the maximum, whereas only one probe could hardly detect the cells. The accessibility map based on the results obtained can be extrapolated to other yeasts, as shown experimentally with 27 additional species (14 ascomycetes and 13 basidiomycetes). This work contributes to a more rational design of species-specific probes for yeast identification and monitoring.
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