ArticleApplied and environmental microbiology2003
Cell culture-Taqman PCR assay for evaluation of Cryptosporidium parvum disinfection.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water.Methods in molecular biology (Clifton, N.J.) · 2026Article
- The Synergistic Effect of Tanabesol and Calcium Hydroxide onInternational journal of veterinary science and medicine · 2026Article
- Cytotoxic innate intraepithelial lymphocytes control early stages ofFrontiers in immunology · 2023Article
- Putative SET-domain methyltransferases inVirulence · 2022Article
- A Simple Alcohol-based Method of Oocyst Inactivation for Use in the Development of Detection Assays forFood and waterborne parasitology · 2022Article
- Extracts of pine bark (Pinus sylvestris) inhibit Cryptosporidium parvum growth in cell culture.Parasitology research · 2021Article
- Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR.Parasites & vectors · 2019Article
- Assessing viability and infectivity of foodborne and waterborne stages (cysts/oocysts) of Giardia duodenalis, Cryptosporidium spp., and Toxoplasma gondii: a review of methods.Parasite (Paris, France) · 2018Review
- Three-dimensional (3D) culture of adult murine colon as an in vitro model of cryptosporidiosis: Proof of concept.Scientific reports · 2017Article
- The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structures.Open veterinary journal · 2016Article
- Oxidative stress control by apicomplexan parasites.BioMed research international · 2015Review
- Determining fungi rRNA copy number by PCR.Journal of biomolecular techniques : JBT · 2013Article
- Comparison of assays for sensitive and reproducible detection of cell culture-infectious Cryptosporidium parvum and Cryptosporidium hominis in drinking water.Applied and environmental microbiology · 2012Article
- Solar radiation induces non-nuclear perturbations and a false start to regulated exocytosis in Cryptosporidium parvum.PloS one · 2010Article
- Stress-induced Hsp70 gene expression and inactivation of Cryptosporidium parvum oocysts by chlorine-based oxidants.Applied and environmental microbiology · 2010Article
- Pulsed-UV light inactivation of Cryptosporidium parvum.Parasitology research · 2008Article
- Aged HCT-8 cell monolayers support Cryptosporidium parvum infection.Applied and environmental microbiology · 2007Article
- Use of DNA melting simulation software for in silico diagnostic assay design: targeting regions with complex melting curves and confirmation by real-time PCR using intercalating dyes.BMC bioinformatics · 2007Article
- Quantification of in vitro and in vivo Cryptosporidium parvum infection by using real-time PCR.Applied and environmental microbiology · 2006Article
- Inactivation of single-celled Ascaris suum eggs by low-pressure UV radiation.Applied and environmental microbiology · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryptosporidium parvum represents a challenge to the water industry and a threat to public health. In this study, we developed a cell culture-quantitative PCR assay to evaluate the inactivation of C. parvum with disinfectants. The assay was validated by using a range of disinfectants in common use in the water industry, including low-pressure UV light (LP-UV), ozone, mixed oxidants (MIOX), and chlorine. The assay was demonstrated to be reliable and sensitive, with a lower detection limit of a single infectious oocyst. Effective oocyst inactivation was achieved (>2 log(10) units) with LP-UV (20 mJ/cm(2)) or 2 mg of ozone/liter (for 10 min). MIOX and chlorine treatments of oocysts resulted in minimal effective disinfection, with <0.1 log(10) unit being inactivated. These results demonstrate the inability of MIOX to inactivate Cryptosporidium. The assay is a valuable tool for the evaluation of disinfection systems for drinking water and recycled water.
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Registered trials
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