ArticleOncogene2003
Cell cycle inhibition mediated by the outer surface of the C/EBPalpha basic region is required but not sufficient for granulopoiesis.
Article in Oncogene, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- Effect and Mechanism Analysis of PigInternational journal of molecular sciences · 2022Article
- Gain-of-Function Effects of N-Terminal CEBPA Mutations in Acute Myeloid Leukemia.BioEssays : news and reviews in molecular, cellular and developmental biology · 2020Review
- Expression level of CEBPA gene in acute lymphoblastic leukemia individuals.Scientific reports · 2019Article
- Article
- Deficiency of the ribosome biogenesis gene Sbds in hematopoietic stem and progenitor cells causes neutropenia in mice by attenuating lineage progression in myelocytes.Haematologica · 2015Article
- Pharmacological targeting of the Wdr5-MLL interaction in C/EBPα N-terminal leukemia.Nature chemical biology · 2015Article
- C/EBPα in normal and malignant myelopoiesis.International journal of hematology · 2015Review
- CUGBP1 promotes cell proliferation and suppresses apoptosis via down-regulating C/EBPα in human non-small cell lung cancers.Medical oncology (Northwood, London, England) · 2015Article
- Article
- C/EBPα dysregulation in AML and ALL.Critical reviews in oncogenesis · 2011Review
- Elevated PIN1 expression by C/EBPalpha-p30 blocks C/EBPalpha-induced granulocytic differentiation through c-Jun in AML.Leukemia · 2010Article
- Article
- Dysregulation of the C/EBPalpha differentiation pathway in human cancer.Journal of clinical oncology : official journal of the American Society of Clinical Oncology · 2009Review
- Review
- Regulation of apoptotic and growth inhibitory activities of C/EBPalpha in different cell lines.Experimental cell research · 2008Article
- C/EBPalpha binds and activates the PU.1 distal enhancer to induce monocyte lineage commitment.Blood · 2007Article
- Review
- Article
- Article
- The proline-histidine-rich CDK2/CDK4 interaction region of C/EBPalpha is dispensable for C/EBPalpha-mediated growth regulation in vivo.Molecular and cellular biology · 2006Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
CCAAT/enhancer binding protein alpha (C/EBPalpha) transactivates target genes dependent upon DNA binding via its basic region-leucine zipper domain and slows G1 progression by interaction with E2F, cdk2, or cdk4. E2F interacts with the non-DNA-binding surface of the C/EBPalpha basic region and C/EBPalpha residues 1-70 are required for repressing E2F targets, while cdk2 and cdk4 bind residues 177-191. C/EBPalpha-ER induces the 32D cl3 myeloblast cell line to differentiate to granulocytes. C/EBPalpha-ER variants incapable of binding DNA slowed G1, but did not induce early or late granulopoiesis, indicating that cell cycle inhibition as mediated by C/EBPalpha is not sufficient for differentiation. C/EBPalpha-ER variants lacking residues 11-70 or residues 11-70 and 178-200 both slowed the G1 to S transition. C/EBPalpha(GZ)-ER, containing the GCN4 rather than the C/EBPalpha leucine zipper, also slowed G1. In contrast, C/EBPalpha(BRM2)-ER, carrying mutations in the outer surface of the basic region required for interaction with E2F, did not slow G1. C/EBPalpha(BRM2)-ER induced early markers of granulopoiesis much less efficiently than C/EBPalpha-ER and did not direct terminal maturation. Inhibition of G1 progression using mimosine increased induction of late markers by G-CSF. Thus, both DNA binding and cell cycle arrest, mediated by opposite surfaces of the C/EBPalpha basic region, are required for granulopoiesis.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.