ArticleProceedings of the National Academy of Sciences of the United States of America2003
Controlling integration specificity of a yeast retrotransposon.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
62 citing papers in PubMed.
- Accumulation of retrotransposons contributes to W chromosome differentiation in the willow beauty Peribatodes rhomboidaria (Lepidoptera: Geometridae).Scientific reports · 2023Article
- Light and shadow on the mechanisms of integration site selection in yeast Ty retrotransposon families.Current genetics · 2021Review
- Patterns of transposable element variation and clinality in Drosophila.Molecular ecology · 2019Article
- Discovery and Evolution of New Domains in Yeast Heterochromatin Factor Sir4 and Its Partner Esc1.Genome biology and evolution · 2019Article
- Targeting IS608 transposon integration to highly specific sequences by structure-based transposon engineering.Nucleic acids research · 2018Article
- Analysis of Transposable Elements in Coccidioides Species.Journal of fungi (Basel, Switzerland) · 2018Article
- Integration site selection by retroviruses and transposable elements in eukaryotes.Nature reviews. Genetics · 2017Review
- Genome-Wide Estimates of Transposable Element Insertion and Deletion Rates in Drosophila Melanogaster.Genome biology and evolution · 2017Article
- Review
- Retroviral DNA Integration.Chemical reviews · 2016Review
- Review
- Sites of retroviral DNA integration: From basic research to clinical applications.Critical reviews in biochemistry and molecular biology · 2016Review
- Evolution and biology of supernumerary B chromosomes.Cellular and molecular life sciences : CMLS · 2014Review
- Molecular mechanisms of retroviral integration site selection.Nucleic acids research · 2014Article
- Small RNAs, big impact: small RNA pathways in transposon control and their effect on the host stress response.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2013Review
- Mapping yeast transcriptional networks.Genetics · 2013Review
- BET proteins promote efficient murine leukemia virus integration at transcription start sites.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Highly diverse chromoviruses of Beta vulgaris are classified by chromodomains and chromosomal integration.Mobile DNA · 2013Article
- Transposon integration enhances expression of stress response genes.Nucleic acids research · 2013Article
- Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
Authors and funding
4 authors.
Funding
Abstract
Retrotransposons and retroviruses integrate nonrandomly into eukaryotic genomes. For the yeast retrotransposon Ty5, integration preferentially occurs within domains of heterochromatin. Targeting to these locations is determined by interactions between an amino acid sequence motif at the C terminus of Ty5 integrase (IN) called the targeting domain, and the heterochromatin protein Sir4p. Here we show that new Ty5 integration hot spots are created when Sir4p is tethered to ectopic DNA sites. Targeting to sites of tethered Sir4p is abrogated by single amino acid substitutions in either IN or Sir4p that prevent their interaction. Ty5 target specificity can be altered by replacing the IN-targeting domain with other peptide motifs that interact with known protein partners. Integration occurs at high efficiency and in close proximity to DNA sites where the protein partners are tethered. These findings define a mechanism by which retrotransposons shape their host genomes and suggest ways in which retroviral integration can be controlled.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.