ArticleProceedings of the National Academy of Sciences of the United States of America2003
Genomewide view of gene silencing by small interfering RNAs.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
84 citing papers in PubMed, 1 synthesis or guideline pooled it, 286 citations in OpenAlex.
- RNA Drugs and RNA Targets for Small Molecules: Principles, Progress, and Challenges.Pharmacological reviews · 2020Pooled it
- Exploring the potential of RNA interference (RNAi) in mosquito control: from mechanisms to molecular insights.In vitro cellular & developmental biology. Animal · 2026Review
- How the pan-genome is changing crop genomics and improvement.Genome biology · 2021Review
- An efficient method for multigene co-interference by recombinant Bombyx mori nucleopolyhedrovirus.Molecular genetics and genomics : MGG · 2019Article
- Evaluation and control of miRNA-like off-target repression for RNA interference.Cellular and molecular life sciences : CMLS · 2018Review
- Persistence of RNAi-Mediated Knockdown in Drosophila Complicates Mosaic Analysis Yet Enables Highly Sensitive Lineage Tracing.Genetics · 2016Article
- Inhibition of mTOR with everolimus and silencing by vascular endothelial cell growth factor-specific siRNA induces synergistic antitumor activity in multiple myeloma cells.Cancer gene therapy · 2014Article
- siSPOTR: a tool for designing highly specific and potent siRNAs for human and mouse.Nucleic acids research · 2013Article
- Applying gene silencing technology to contraception.Reproduction in domestic animals = Zuchthygiene · 2012Article
- Targeted gene silencing to induce permanent sterility.Reproduction in domestic animals = Zuchthygiene · 2012Article
- Rational design of therapeutic siRNAs: minimizing off-targeting potential to improve the safety of RNAi therapy for Huntington's disease.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Article
- Vigilance and validation: Keys to success in RNAi screening.ACS chemical biology · 2011Review
- Small-interfering RNA-eluting surfaces as a novel concept for intravascular local gene silencing.Molecular medicine (Cambridge, Mass.) · 2011Article
- Quantitative proteomics identification of phosphoglycerate mutase 1 as a novel therapeutic target in hepatocellular carcinoma.Molecular cancer · 2010Article
- Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application.Nature reviews. Drug discovery · 2010Review
- Activity of TAR in inducible inhibition of HIV replication by foamy virus vector expressing siRNAs under the control of HIV LTR.Virus research · 2009Article
- Article
- Off-target effects of siRNA specific for GFP.BMC molecular biology · 2008Article
- Experimental validation of the importance of seed complement frequency to siRNA specificity.RNA (New York, N.Y.) · 2008Article
- A dermal HOX transcriptional program regulates site-specific epidermal fate.Genes & development · 2008Article
24 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) is an evolutionarily conserved mechanism in plant and animal cells that directs the degradation of messenger RNAs homologous to short double-stranded RNAs termed small interfering RNA (siRNA). The ability of siRNA to direct gene silencing in mammalian cells has raised the possibility that siRNA might be used to investigate gene function in a high throughput fashion or to modulate gene expression in human diseases. The specificity of siRNA-mediated silencing, a critical consideration in these applications, has not been addressed on a genomewide scale. Here we show that siRNA-induced gene silencing of transient or stably expressed mRNA is highly gene-specific and does not produce secondary effects detectable by genomewide expression profiling. A test for transitive RNAi, extension of the RNAi effect to sequences 5' of the target region that has been observed in Caenorhabditis elegans, was unable to detect this phenomenon in human cells.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.