ArticleThe Journal of physiology2003
C-type inactivation involves a significant decrease in the intracellular aqueous pore volume of Kv1.4 K+ channels expressed in Xenopus oocytes.
Article in The Journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Sweetening K-channels: what sugar taught us about permeation and gating.Frontiers in molecular biosciences · 2023Review
- A folding reaction at the C-terminal domain drives temperature sensing in TRPM8 channels.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Gating-induced large aqueous volumetric remodeling and aspartate tolerance in the voltage sensor domain of Shaker KProceedings of the National Academy of Sciences of the United States of America · 2018Article
- The Effects of 4-Hydroxybenzoic Acid Identified from Bamboo (The Malaysian journal of medical sciences : MJMS · 2018Article
- Are Aquaporins the Missing Transmembrane Osmosensors?The Journal of membrane biology · 2015Review
- Synaptotagmin I delays the fast inactivation of Kv1.4 channel through interaction with its N-terminus.Molecular brain · 2014Article
- Mercury-sensitive water channels as possible sensors of water potentials in pollen.Journal of experimental botany · 2013Article
- Interaction of the S6 proline hinge with N-type and C-type inactivation in Kv1.4 channels.Biophysical journal · 2012Article
- A model of the interaction between N-type and C-type inactivation in Kv1.4 channels.Biophysical journal · 2011Article
- A molecular switch between the outer and the inner vestibules of the voltage-gated Na+ channel.The Journal of biological chemistry · 2010Article
- Closed-state inactivation in Kv4.3 isoforms is differentially modulated by protein kinase C.American journal of physiology. Cell physiology · 2009Article
- Slow inactivation in Shaker K channels is delayed by intracellular tetraethylammonium.The Journal of general physiology · 2008Article
- Modification of K+ channel-drug interactions by ancillary subunits.The Journal of physiology · 2008Review
- Speeding the recovery from ultraslow inactivation of voltage-gated Na+ channels by metal ion binding to the selectivity filter: a foot-on-the-door?Biophysical journal · 2007Article
- Transient outward potassium current, 'Ito', phenotypes in the mammalian left ventricle: underlying molecular, cellular and biophysical mechanisms.The Journal of physiology · 2005Review
- Time- and voltage-dependent components of Kv4.3 inactivation.Biophysical journal · 2005Article
- Slow inactivation in voltage gated potassium channels is insensitive to the binding of pore occluding peptide toxins.Biophysical journal · 2005Article
- Tryptophan substitution of a putative D4S6 gating hinge alters slow inactivation in cardiac sodium channels.Biophysical journal · 2005Article
- Surface changes of the mechanosensitive channel MscS upon its activation, inactivation, and closing.Biophysical journal · 2005Article
- Activation properties of Kv4.3 channels: time, voltage and [K+]o dependence.The Journal of physiology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Channels are water-filled membrane-spanning proteins, which undergo conformational changes as they gate, i.e. open or close. These conformational changes affect both the shape of the channel and the volume of the water-filled pore. We measured the changes in pore volume associated with activation, deactivation, C-type inactivation and recovery in an N-terminal-deleted mutant of the Kv1.4 K+ channel (Kv1.4DeltaN) expressed in Xenopus oocytes. We used giant-patch and cut-open oocyte voltage clamp techniques and applied solutes which are too large to enter the pore mouth to exert osmotic pressure and thus favour smaller pore volume conformations. Applied intracellular osmotic pressure (300 mM sucrose) sped inactivation (time constants (tauinactivation): control, 0.66 +/- 0.09 s; hyperosmotic solution, 0.29 +/- 0.04 s; n = 5, P < 0.01), sped deactivation (taudeactivation: control, 18.8 +/- 0.94 ms; hyperosmotic solution, 8.01 +/- 1.92 ms; n = 5, P < 0.01), and slowed activation (tauactivation: control, 1.04 +/- 0.05 ms; hyperosmotic solution, 1.96 +/- 0.31 ms; n = 5, P < 0.01). These effects were reversible and solute independent. We estimated the pore volume change on inactivation to be about 4500 A3. Osmotic pressure had no effect when applied extracellularly. These data suggest that the intracellular side of the pore closes during C-type inactivation and the volume change is similar to that associated with activation or deactivation. This is also similar to the pore volume estimated from the crystal structure of KcsA and MthK K+ channels. Intracellular osmotic pressure also strongly inhibited re-opening currents associated with recovery from inactivation, which is consistent with a physical similarity between the C-type inactivated and resting closed state.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.