Evidence map›Paper›PMID 12730186›Full record

ArticleJournal of bacteriology2003

Differential expression of the components of the two alkane hydroxylases from Pseudomonas aeruginosa.

Mercedes M Marín, Luis Yuste, Fernando Rojo

Open access · greenAbstract read
In one paragraph

Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
1.4field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 98 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Mercedes M MarínDepartamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Campus de la Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
Luis Yuste
Fernando Rojo
Universidad Autónoma de Madrid · ESCentro Nacional de Biotecnología · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Oxidation of n-alkanes in bacteria is normally initiated by an enzyme system formed by a membrane-bound alkane hydroxylase and two soluble proteins, rubredoxin and rubredoxin reductase. Pseudomonas aeruginosa strains PAO1 and RR1 contain genes encoding two alkane hydroxylases (alkB1 and alkB2), two rubredoxins (alkG1 and alkG2), and a rubredoxin reductase (alkT). We have localized the promoters for these genes and analyzed their expression under different conditions. The alkB1 and alkB2 genes were preferentially expressed at different moments of the growth phase; expression of alkB2 was highest during the early exponential phase, while alkB1 was induced at the late exponential phase, when the growth rate decreased. Both genes were induced by C(10) to C(22)/C(24) alkanes but not by their oxidation derivatives. However, the alkG1, alkG2, and alkT genes were expressed at constant levels in both the absence and presence of alkanes.

Indexed as

AlkanesBacterial ProteinsBase SequenceCell DivisionCytochrome P-450 CYP4ACytochrome P-450 Enzyme SystemDNA-Binding ProteinsGene Expression Regulation, BacterialGene Expression Regulation, EnzymologicMixed Function OxygenasesMolecular Sequence DataMultigene FamilyNADH, NADPH OxidoreductasesOxidation-ReductionPromoter Regions, GeneticPseudomonas aeruginosaAlkanesBacterial ProteinsCytochrome P-450 CYP4ACytochrome P-450 Enzyme SystemDNA-Binding Proteinsguanosine-3',5'-bis(diphosphate) 3'-pyrophosphataseLasR protein, Pseudomonas aeruginosaMixed Function OxygenasesNADH, NADPH OxidoreductasesPyrophosphatasesrhiR protein, Rhizobium leguminosarumrubredoxin-NAD+ reductaseRubredoxinsSigma Factorsigma factor KatF protein, BacteriaTrans-Activators

Identifiers

PMID12730186
PMCPMC154056
OpenAlexW2129360612

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.