ArticleGenome research2003
Selecting open reading frames from DNA.
Article in Genome research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 49 citations in OpenAlex.
- Review
- Bacteriophages as Potential Tools for Use in Antimicrobial Therapy and Vaccine Development.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Defining theFrontiers in microbiology · 2020Article
- An efficient ORF selection system for DNA fragment libraries based on split beta-lactamase complementation.PloS one · 2020Article
- Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing.Journal of visualized experiments : JoVE · 2018Article
- An att site-based recombination reporter system for genome engineering and synthetic DNA assembly.BMC biotechnology · 2017Article
- Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display.Frontiers in microbiology · 2016Review
- Identification of novel proteins binding the AU-rich element of α-prothymosin mRNA through the selection of open reading frames (RIDome).RNA biology · 2015Article
- Interaction analysis through proteomic phage display.BioMed research international · 2014Review
- Oligopeptide m13 phage display in pathogen research.Viruses · 2013Review
- A novel helper phage enabling construction of genome-scale ORF-enriched phage display libraries.PloS one · 2013Article
- Construction of a rationally designed antibody platform for sequencing-assisted selection.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- ORF phage display to identify cellular proteins with different functions.Methods (San Diego, Calif.) · 2012Article
- Experimental mapping of soluble protein domains using a hierarchical approach.Nucleic acids research · 2011Article
- A high-throughput immobilized bead screen for stable proteins and multi-protein complexes.Protein engineering, design & selection : PEDS · 2011Article
- Filtering "genic" open reading frames from genomic DNA samples for advanced annotation.BMC genomics · 2011Article
- Autoantigen discovery with a synthetic human peptidome.Nature biotechnology · 2011Article
- Design and screening of M13 phage display cDNA libraries.Molecules (Basel, Switzerland) · 2011Review
- Identification of Hnrph3 as an autoantigen for acute anterior uveitis.Clinical immunology (Orlando, Fla.) · 2011Article
- Rapid interactome profiling by massive sequencing.Nucleic acids research · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a method to select DNA encoding functional open reading frames (ORFs) from noncoding DNA within the context of a specific vector. Phage display has been used as an example, but any system requiring DNA encoding protein fragments, for example, the yeast two-hybrid system, could be used. By cloning DNA fragments upstream of a fusion gene, consisting of the beta-lactamase gene flanked by lox recombination sites, which is, in turn, upstream of gene 3 from fd phage, only those clones containing DNA fragments encoding ORFs confer ampicillin resistance and survive. After selection, the beta-lactamase gene can be removed by Cre recombinase, leaving a standard phage display vector with ORFs fused to gene 3. This vector has been tested on a plasmid containing tissue transglutaminase. All surviving clones analyzed by sequencing were found to contain ORFs, of which 83% were localized to known genes, and at least 80% produced immunologically detectable polypeptides. Use of a specific anti-tTG monoclonal antibody allowed the identification of clones containing the correct epitope. This approach could be applicable to the efficient selection of random ORFs representing the coding potential of whole organisms, and their subsequent downstream use in a number of different systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.