ArticleThe EMBO journal2003
Regions of GAL4 critical for binding to a promoter in vivo revealed by a visual DNA-binding analysis.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Transcription factor clusters enable target search but do not contribute to target gene activation.Nucleic acids research · 2023Article
- Article
- Insights into Bidirectional Gene Expression Control Using the Canonical GAL1/GAL10 Promoter.Cell reports · 2018Article
- A Precise Genome Editing Method Reveals Insights into the Activity of Eukaryotic Promoters.Cell reports · 2017Article
- Rapid GAL gene switch of Saccharomyces cerevisiae depends on nuclear Gal3, not nucleocytoplasmic trafficking of Gal3 and Gal80.Genetics · 2011Article
- A general mechanism for network-dosage compensation in gene circuits.Science (New York, N.Y.) · 2010Article
- Gene activation by dissociation of an inhibitor from a transcriptional activation domain.Molecular and cellular biology · 2009Article
- Gal80 dimerization and the yeast GAL gene switch.Genetics · 2005Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Binding of transcriptional activators to specific sites on DNA, mediated by their DNA-binding domain, is a key regulatory point in transcriptional regulation. With a GFP-based microscopic assay, we investigated how the prototypical activator GAL4 effectively binds to a promoter in living yeast cells. We show that GAL4 relies on a previously unrevealed mechanism involving 'DNA-binding enhancers' (DBEs), the regions of GAL4 that assist DNA-binding domain association with DNA. GAL4 contains two DBEs, one, but not the other, physically overlapping the principal transcriptional activation domain. Either of the DBEs, however, can function independently of transcriptional activation, indicating a discrete mechanism responsible for DNA-binding enhancement. The effect of DBEs, while not limited to natural target promoters, is still not universal and can be profoundly affected by the binding-site context. The GAL4 DBEs can also enhance promoter binding of an unrelated DNA-binding domain, and possibly represent a new modular functional unit responsible for effective binding of diverse regulatory factors to DNA in vivo.
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Registered trials
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