ArticleThe Plant cell2003
Rha1, an Arabidopsis Rab5 homolog, plays a critical role in the vacuolar trafficking of soluble cargo proteins.
Article in The Plant cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
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Who cites it
99 citing papers in PubMed, 198 citations in OpenAlex.
- Canonical Rab5 GTPases Confer Heat-Stress Resilience to Pollen Germination.Plant, cell & environment · 2026Article
- A viral protein diverts the endocytic VPS9a-Rab5 pathway to promote virus infection in plants.Science advances · 2026Article
- Post-Golgi Trafficking in Plant Cells.Sub-cellular biochemistry · 2026Review
- Rab5 GTPases mediate the targeting of ROP signalling to establish polarity for pollen germination.Nature plants · 2025Article
- Protein Prenylation in Plants: Mechanisms and Functional Implications.Plants (Basel, Switzerland) · 2025Review
- Characterization of the small Arabidopsis thaliana GTPase and ADP-ribosylation factor-like 2 protein TITAN 5.Journal of cell science · 2024Article
- Cold tolerance SNPs and candidate gene mining in the soybean germination stage based on genome-wide association analysis.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Characterization of the smallbioRxiv : the preprint server for biology · 2024Article
- Expression and localization of two β-carbonic anhydrases inFrontiers in plant science · 2024Article
- Rab GTPases, tethers, and SNAREs work together to regulateFrontiers in plant science · 2023Review
- RAB GTPases and SNAREs at the trans-Golgi network in plants.Journal of plant research · 2022Review
- An efficient and broadly applicable method for transient transformation of plants using vertically aligned carbon nanofiber arrays.Frontiers in plant science · 2022Article
- Vacuoles in Bryophytes: Properties, Biogenesis, and Evolution.Frontiers in plant science · 2022Review
- A robust and practical CRISPR/crRNA screening system for soybean cultivar editing using LbCpf1 ribonucleoproteins.Plant cell reports · 2021Article
- Plant RABs: Role in Development and in Abiotic and Biotic Stress Responses.Current genomics · 2021Review
- Heat Stress-Dependent Association of Membrane Trafficking Proteins With mRNPs Is Selective.Frontiers in plant science · 2021Article
- Organelle Visualization With Multicolored Fluorescent Markers in Bamboo.Frontiers in plant science · 2021Article
- Salt-induced expression of intracellular vesicle trafficking genes, CaRab-GTP, and their association with NaBMC plant biology · 2020Article
- Transcriptome-wide identification and characterization of the Rab GTPase family in mango.Molecular biology reports · 2020Article
- A Diverse Membrane Interaction Network for Plant Multivesicular Bodies: Roles in Proteins Vacuolar Delivery and Unconventional Secretion.Frontiers in plant science · 2020Review
39 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rab proteins are members of the Ras superfamily of small GTP binding proteins and play important roles in various intracellular trafficking steps. We investigated the role of Rha1, an Arabidopsis Rab5 homolog, in intracellular trafficking in Arabidopsis protoplasts. In the presence of a dominant-negative mutant of Rha1, soluble vacuolar cargo proteins such as sporamin:green fluorescent protein (Spo:GFP) and Arabidopsis aleurain like protein:GFP are not delivered to the central vacuole; instead, they accumulate as a diffuse or punctate staining pattern within the cell. Spo:GFP at the punctate stains observed in the presence of hemagglutinin:Rha1[S24N] is colocalized with endogenous vacuolar sorting receptor (VSR(At-1)), which is known to localize primarily to the prevacuolar compartment, whereas Spo:GFP in the diffuse pattern is associated with tonoplasts. Furthermore, expression of Rha1[S24N] causes the secretion of a portion of the vacuolar proteins into medium. However, the inhibitory effect of Rha1[S24N] on vacuolar trafficking is relieved partially by coexpressed wild-type Rha1. Based on these results, we propose that Rha1 plays a critical role in the trafficking of soluble cargoes from the prevacuolar compartment to the central vacuole.
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