ArticleMolecular and cellular biology2003
Constitutive E2F1 overexpression delays endochondral bone formation by inhibiting chondrocyte differentiation.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
- Absence of E2f1 Negates Pro-osteogenic Impacts of p21 Absence.Calcified tissue international · 2024Article
- Functional Duality of Chondrocyte Hypertrophy and Biomedical Application Trends in Osteoarthritis.Pharmaceutics · 2021Review
- E2F1 promotes hepatic gluconeogenesis and contributes to hyperglycemia during diabetes.Molecular metabolism · 2018Article
- Genetic Markers Can Predict Chondrogenic Differentiation Potential in Bone Marrow-Derived Mesenchymal Stromal Cells.Stem cells international · 2018Article
- p21BMC musculoskeletal disorders · 2017Article
- Gene expression changes in damaged osteoarthritic cartilage identify a signature of non-chondrogenic and mechanical responses.Osteoarthritis and cartilage · 2016Article
- Study of differential effects of TGF-beta3/BMP2 on chondrogenesis in MSC cells by gene microarray data analysis.Molecular and cellular biochemistry · 2014Article
- Article
- Loss of pRB and p107 disrupts cartilage development and promotes enchondroma formation.Oncogene · 2013Article
- Defective skeletal muscle growth in lamin A/C-deficient mice is rescued by loss of Lap2α.Human molecular genetics · 2013Article
- A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop.Nucleic acids research · 2013Article
- Article
- p107 in the public eye: an Rb understudy and more.Cell division · 2010Article
- Article
- Transcriptional networks controlling chondrocyte proliferation and differentiation during endochondral ossification.Pediatric nephrology (Berlin, Germany) · 2010Review
- The retinoblastoma protein tumor suppressor is important for appropriate osteoblast differentiation and bone development.Molecular cancer research : MCR · 2008Article
- A role for a lithium-inhibited Golgi nucleotidase in skeletal development and sulfation.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Cooperation between p27 and p107 during endochondral ossification suggests a genetic pathway controlled by p27 and p130.Molecular and cellular biology · 2007Article
- E2F1 stability is regulated by a novel-PKC/p38beta MAP kinase signaling pathway during keratinocyte differentiation.Oncogene · 2006Article
- Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Longitudinal bone growth results from endochondral ossification, a process that requires proliferation and differentiation of chondrocytes. It has been shown that proper endochondral bone formation is critically dependent on the retinoblastoma family members p107 and p130. However, the precise functional roles played by individual E2F proteins remain poorly understood. Using both constitutive and conditional E2F1 transgenic mice, we show that ubiquitous transgene-driven expression of E2F1 during embryonic development results in a dwarf phenotype and significantly reduced postnatal viability. Overexpression of E2F1 disturbs chondrocyte maturation, resulting in delayed endochondral ossification, which is characterized by reduced hypertrophic zones and disorganized growth plates. Employing the chondrogenic cell line ATDC5, we investigated the effects of enforced E2F expression on the different phases of chondrocyte maturation that are normally required for endochondral ossification. Ectopic E2F1 expression strongly inhibits early- and late-phase differentiation of ATDC5 cells, accompanied by diminished cartilage nodule formation as well as decreased type II collagen, type X collagen, and aggrecan gene expression. In contrast, overexpression of E2F2 or E2F3a results in only a marginal delay of chondrocyte maturation, and increased E2F4 levels have no effect. These data are consistent with the notion that E2F1 is a regulator of chondrocyte differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.