ArticleMolecular and cellular biology2003
Nonmuscle myosin promotes cytoplasmic localization of PBX.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Nonmuscle myosin IIB is a driver of cellular reprogramming.Molecular biology of the cell · 2023Article
- Research progress of PBX1 in developmental and regenerative medicine.International journal of medical sciences · 2023Review
- The regulation of PBXs and their emerging role in cancer.Journal of cellular and molecular medicine · 2022Review
- Posttranslational Modifications in Conserved Transcription Factors: A Survey of the TALE-Homeodomain Superclass in Human and Mouse.Frontiers in cell and developmental biology · 2021Review
- Mapping the native interaction surfaces of PREP1 with PBX1 by cross-linking mass-spectrometry and mutagenesis.Scientific reports · 2020Article
- Dynamic expression patterns of Irx3 and Irx5 during germline nest breakdown and primordial follicle formation promote follicle survival in mouse ovaries.PLoS genetics · 2018Article
- Biochemistry of the tale transcription factors PREP, MEIS, and PBX in vertebrates.Developmental dynamics : an official publication of the American Association of Anatomists · 2014Review
- Hox regulation of transcription: more complex(es).Developmental dynamics : an official publication of the American Association of Anatomists · 2014Review
- Karyopherins in nuclear transport of homeodomain proteins during development.Biochimica et biophysica acta · 2011Review
- Transcriptional activation by MEIS1A in response to protein kinase A signaling requires the transducers of regulated CREB family of CREB co-activators.The Journal of biological chemistry · 2009Article
- Hox specificity unique roles for cofactors and collaborators.Current topics in developmental biology · 2009Review
- Myosin IIC: a third molecular motor driving neuronal dynamics.Molecular biology of the cell · 2008Article
- Drosophila Myosin II, Zipper, is essential for ommatidial rotation.Developmental biology · 2007Article
- Myosin-IIA heavy-chain phosphorylation regulates the motility of MDA-MB-231 carcinoma cells.Molecular biology of the cell · 2007Article
- A balance between two nuclear localization sequences and a nuclear export sequence governs extradenticle subcellular localization.Genetics · 2007Article
- Pre-B-cell leukemia transcription factor 1 regulates expression of valosin-containing protein, a gene involved in cancer growth.The American journal of pathology · 2007Article
- Nonmuscle myosin IIA-dependent force inhibits cell spreading and drives F-actin flow.Biophysical journal · 2006Article
- The protein ENH is a cytoplasmic sequestration factor for Id2 in normal and tumor cells from the nervous system.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- A central role of Arabidopsis thaliana ovate family proteins in networking and subcellular localization of 3-aa loop extension homeodomain proteins.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- FOXC1 transcriptional regulatory activity is impaired by PBX1 in a filamin A-mediated manner.Molecular and cellular biology · 2005Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the absence of MEIS family proteins, two mechanisms are known to restrict the PBX family of homeodomain (HD) transcription factors to the cytoplasm. First, PBX is actively exported from the nucleus via a CRM1-dependent pathway. Second, nuclear localization signals (NLSs) within the PBX HD are masked by intramolecular contacts. In a screen to identify additional proteins directing PBX subcellular localization, we identified a fragment of murine nonmuscle myosin II heavy chain B (NMHCB). The interaction of NMHCB with PBX was verified by coimmunoprecipitation, and immunofluorescence staining revealed colocalization of NMHCB with cytoplasmic PBX in the mouse embryo distal limb bud. The interaction domain in PBX mapped to a conserved PBC-B region harboring a potential coiled-coil structure. In support of the cytoplasmic retention function, the NMHCB fragment competes with MEIS1A to redirect PBX, and the fly PBX homologue EXD, to the cytoplasm of mammalian and insect cells. Interestingly, MEIS1A also localizes to the cytoplasm in the presence of the NMHCB fragment. These activities are largely independent of nuclear export. We show further that the subcellular localization of EXD is deregulated in Drosophila zipper mutants that are depleted of nonmuscle myosin heavy chain. This study reveals a novel and evolutionarily conserved mechanism controlling the subcellular distribution of PBX and EXD proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.