ArticleMolecular and cellular biology2003
Replication of the chicken beta-globin locus: early-firing origins at the 5' HS4 insulator and the rho- and betaA-globin genes show opposite epigenetic modifications.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 59 citations in OpenAlex.
- Determination of human DNA replication origin position and efficiency reveals principles of initiation zone organisation.Nucleic acids research · 2022Article
- Various short autonomously replicating sequences from the yeastCurrent research in microbial sciences · 2021Article
- Alternative DNA StructuresMicrobiology and molecular biology reviews : MMBR · 2021Review
- Evolution of replication origins in vertebrate genomes: rapid turnover despite selective constraints.Nucleic acids research · 2019Article
- Article
- Local epigenetic reprogramming induced by G-quadruplex ligands.Nature chemistry · 2017Article
- Peaks cloaked in the mist: the landscape of mammalian replication origins.The Journal of cell biology · 2015Review
- The hunt for origins of DNA replication in multicellular eukaryotes.F1000prime reports · 2015Review
- Chromatin structure and replication origins: determinants of chromosome replication and nuclear organization.Journal of molecular biology · 2014Review
- Epigenetic landscape for initiation of DNA replication.Chromosoma · 2014Review
- The spatiotemporal program of DNA replication is associated with specific combinations of chromatin marks in human cells.PLoS genetics · 2014Article
- G4 motifs affect origin positioning and efficiency in two vertebrate replicators.The EMBO journal · 2014Article
- High-resolution analysis of DNA synthesis start sites and nucleosome architecture at efficient mammalian replication origins.The EMBO journal · 2013Article
- RNAP-II molecules participate in the anchoring of the ORC to rDNA replication origins.PloS one · 2013Article
- Propagation of histone marks and epigenetic memory during normal and interrupted DNA replication.Cellular and molecular life sciences : CMLS · 2012Review
- USF binding sequences from the HS4 insulator element impose early replication timing on a vertebrate replicator.PLoS biology · 2012Article
- Genome-wide mapping of Arabidopsis thaliana origins of DNA replication and their associated epigenetic marks.Nature structural & molecular biology · 2011Article
- Genomic approaches to the initiation of DNA replication and chromatin structure reveal a complex relationship.Briefings in functional genomics · 2011Review
- Epigenetic instability due to defective replication of structured DNA.Molecular cell · 2010Article
- A multiprotein complex necessary for both transcription and DNA replication at the β-globin locus.The EMBO journal · 2010Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromatin structure is believed to exert a strong effect on replication origin function. We have studied the replication of the chicken beta-globin locus, whose chromatin structure has been extensively characterized. This locus is delimited by hypersensitive sites (HSs) that mark the position of insulator elements. A stretch of condensed chromatin and another HS separate the beta-globin domain from an adjacent folate receptor (FR) gene. We demonstrate here that in erythroid cells that express the FR but not the globin genes, replication initiates at four sites within the beta-globin domain, one at the 5' HS4 insulator and the other three near the rho- and beta(A)-globin genes. Three origins consist of G+C-rich sequences enriched in CpG dinucleotides. The fourth origin is A+T rich. Together with previous work, these data reveal that the insulator origin has unmethylated CpGs, hyperacetylated histones H3 and H4, and lysine 4-methylated histone H3. In contrast, opposite modifications are observed at the other G+C-rich origins. We also show that the whole region, including the stretch of condensed chromatin, replicates early in S phase in these cells. Therefore, different early-firing origins within the same locus may have opposite patterns of epigenetic modifications. The role of insulator elements in DNA replication is discussed.
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