ArticleMolecular and cellular biology2003
Functional relation among RecQ family helicases RecQL1, RecQL5, and BLM in cell growth and sister chromatid exchange formation.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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50 citing papers in PubMed, 87 citations in OpenAlex.
- The proofreading exonuclease of leading-strand DNA polymerase epsilon prevents replication fork collapse at broken template strands.Nucleic acids research · 2023Article
- Review
- Biochemical Properties of Naturally Occurring Human Bloom Helicase Variants.bioRxiv : the preprint server for biology · 2023Article
- Article
- RECON syndrome is a genome instability disorder caused by mutations in the DNA helicase RECQL1.The Journal of clinical investigation · 2022Article
- Review
- Review
- Bloom syndrome: research and data priorities for the development of precision medicine as identified by some affected families.Cold Spring Harbor molecular case studies · 2018Review
- BLM helicase regulates DNA repair by counteracting RAD51 loading at DNA double-strand break sites.The Journal of cell biology · 2017Article
- Human RECQ Helicase Pathogenic Variants, Population Variation and "Missing" Diseases.Human mutation · 2017Article
- Exploring Quantitative Yeast Phenomics with Single-Cell Analysis of DNA Damage Foci.Cell systems · 2016Article
- A Comparative Study of G-Quadruplex Unfolding and DNA Reeling Activities of Human RECQ5 Helicase.Biophysical journal · 2016Article
- Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines.Genes to cells : devoted to molecular & cellular mechanisms · 2015Article
- Tumor suppressor RecQL5 controls recombination induced by DNA crosslinking agents.Biochimica et biophysica acta · 2014Article
- Tipin functions in the protection against topoisomerase I inhibitor.The Journal of biological chemistry · 2014Article
- Human RECQ5 helicase promotes repair of DNA double-strand breaks by synthesis-dependent strand annealing.Nucleic acids research · 2014Article
- Human RecQ helicases in DNA repair, recombination, and replication.Annual review of biochemistry · 2014Review
- RECQL5 plays co-operative and complementary roles with WRN syndrome helicase.Nucleic acids research · 2013Article
- Human RECQL5 participates in the removal of endogenous DNA damage.Molecular biology of the cell · 2012Article
- Genetically engineered mouse models and human osteosarcoma.Clinical sarcoma research · 2012Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human RECQL1 and RECQL5 belong to the RecQ family that includes Bloom syndrome, Werner syndrome, and Rothmund-Thomson syndrome causative genes. Cells derived from individuals suffering from these syndromes show significant levels of genomic instability. However, neither RECQL1 nor RECQL5 has been related to a disease, and nothing is known about the functions of RecQL1 and RecQL5. We generated here RECQL1(-/-), RECQL5(-/-), RECQL1(-/-)/RECQL5(-/-), RECQL1(-/-)/BLM(-/-), and RECQL5(-/-)/BLM(-/-) cells from chicken B-lymphocyte line DT40 cells. Although BLM(-/-) DT40 cells showed a slow-growth phenotype, a higher sensitivity to methyl methanesulfonate than the wild type, and an approximately 10-fold increase in the frequency of sister chromatid exchange (SCE) compared to wild-type cells, RECQL1(-/-), RECQL5(-/-), and RECQL1(-/-)/RECQL5(-/-) cells showed no significant difference from the wild-type cells in growth, sensitivity to DNA-damaging agents, and the frequency of SCE. However, both RECQL1(-/-)/BLM(-/-) and RECQL5(-/-)/BLM(-/-) cells grew more slowly than BLM(-/-) cells because of the increase in the population of dead cells, indicating that RecQL1 and RecQL5 are somehow involved in cell viability under the BLM function-impaired condition. Surprisingly, RECQL5(-/-)/BLM(-/-) cells showed a higher frequency of SCE than BLM(-/-) cells, indicating that RecQL5 suppresses SCE under the BLM function-impaired condition.
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