ArticleMolecular and cellular biology2003
The U1 snRNP base pairs with the 5' splice site within a penta-snRNP complex.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 60 citations in OpenAlex.
- Deletions of singular U1 snRNA gene significantly interfere with transcription and 3'-end mRNA formation.PLoS genetics · 2023Article
- Introns: the "dark matter" of the eukaryotic genome.Frontiers in genetics · 2023Review
- A genetic screen in C. elegans reveals roles for KIN17 and PRCC in maintaining 5' splice site identity.PLoS genetics · 2022Article
- Alternative Splicing Shapes the Phenotype of a Mutation in BBS8 To Cause Nonsyndromic Retinitis Pigmentosa.Molecular and cellular biology · 2015Article
- Vemurafenib-resistant BRAF selects alternative branch points different from its wild-type BRAF in intron 8 for RNA splicing.Cell & bioscience · 2015Article
- A day in the life of the spliceosome.Nature reviews. Molecular cell biology · 2014Review
- The nuclear cap-binding complex interacts with the U4/U6·U5 tri-snRNP and promotes spliceosome assembly in mammalian cells.RNA (New York, N.Y.) · 2013Article
- A unique spatial arrangement of the snRNPs within the native spliceosome emerges from in silico studies.Structure (London, England : 1993) · 2012Article
- Structure of spliceosomal ribonucleoproteins.F1000 biology reports · 2010Article
- A mechanism for incorporation of galectin-3 into the spliceosome through its association with U1 snRNP.Biochemistry · 2009Article
- The assembly of a spliceosomal small nuclear ribonucleoprotein particle.Nucleic acids research · 2008Review
- Requirements for gene silencing mediated by U1 snRNA binding to a target sequence.Nucleic acids research · 2008Article
- Splicing-independent recruitment of spliceosomal small nuclear RNPs to nascent RNA polymerase II transcripts.The Journal of cell biology · 2007Article
- The abundance of the spliceosomal snRNPs is not limiting the splicing of U12-type introns.RNA (New York, N.Y.) · 2006Article
- Functional spliceosomal A complexes can be assembled in vitro in the absence of a penta-snRNP.RNA (New York, N.Y.) · 2006Article
- Arrested yeast splicing complexes indicate stepwise snRNP recruitment during in vivo spliceosome assembly.RNA (New York, N.Y.) · 2006Article
- Multiple genetic and biochemical interactions of Brr2, Prp8, Prp31, Prp1 and Prp4 kinase suggest a function in the control of the activation of spliceosomes in Schizosaccharomyces pombe.Current genetics · 2005Review
- Retroviral splicing suppressor sequesters a 3' splice site in a 50S aberrant splicing complex.Molecular and cellular biology · 2005Article
- Proximity of the U12 snRNA with both the 5' splice site and the branch point during early stages of spliceosome assembly.Molecular and cellular biology · 2005Article
- Prp8 protein: at the heart of the spliceosome.RNA (New York, N.Y.) · 2005Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recognition of the 5' splice site is an important step in mRNA splicing. To examine whether U1 approaches the 5' splice site as a solitary snRNP or as part of a multi-snRNP complex, we used a simplified in vitro system in which a short RNA containing the 5' splice site sequence served as a substrate in a binding reaction. This system allowed us to study the interactions of the snRNPs with the 5' splice site without the effect of other cis-regulatory elements of precursor mRNA. We found that in HeLa cell nuclear extracts, five spliceosomal snRNPs form a complex that specifically binds the 5' splice site through base pairing with the 5' end of U1. This system can accommodate RNA-RNA rearrangements in which U5 replaces U1 binding to the 5' splice site, a process that occurs naturally during the splicing reaction. The complex in which U1 and the 5' splice site are base paired sediments in the 200S fraction of a glycerol gradient together with all five spliceosomal snRNPs. This fraction is functional in mRNA spliceosome assembly when supplemented with soluble nuclear proteins. The results argue that U1 can bind the 5' splice site in a mammalian preassembled penta-snRNP complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.