ArticleMolecular and cellular biology2003
Rad53 checkpoint kinase phosphorylation site preference identified in the Swi6 protein of Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 53 citations in OpenAlex.
- Rad53 regulates the lifetime of Rdh54 at homologous recombination intermediates.Nucleic acids research · 2023Article
- Hydroxyurea and inactivation of checkpoint kinase MEC1 inhibit transcription termination and pre-mRNA cleavage at polyadenylation sites in budding yeast.Scientific reports · 2023Article
- Prevalent and dynamic binding of the cell cycle checkpoint kinase Rad53 to gene promoters.eLife · 2022Article
- Effects of Non-Thermal Plasma on YeastInternational journal of molecular sciences · 2021Review
- DNA damage kinase signaling: checkpoint and repair at 30 years.The EMBO journal · 2019Review
- DNA damage response activates respiration and thereby enlarges dNTP pools to promote cell survival in budding yeast.The Journal of biological chemistry · 2019Article
- Helicase Subunit Cdc45 Targets the Checkpoint Kinase Rad53 to Both Replication Initiation and Elongation Complexes after Fork Stalling.Molecular cell · 2019Article
- Prevention of DNA Rereplication Through a Meiotic Recombination Checkpoint Response.G3 (Bethesda, Md.) · 2016Article
- Protein kinase C controls activation of the DNA integrity checkpoint.Nucleic acids research · 2014Article
- Protein kinase CK2 holoenzyme promotes start-specific transcription in Saccharomyces cerevisiae.Eukaryotic cell · 2013Article
- Checkpoint kinases regulate a global network of transcription factors in response to DNA damage.Cell reports · 2013Article
- Eukaryotic DNA damage checkpoint activation in response to double-strand breaks.Cellular and molecular life sciences : CMLS · 2012Review
- DNA replication stress differentially regulates G1/S genes via Rad53-dependent inactivation of Nrm1.The EMBO journal · 2012Article
- Ixr1 is required for the expression of the ribonucleotide reductase Rnr1 and maintenance of dNTP pools.PLoS genetics · 2011Article
- A strategy for interaction site prediction between phospho-binding modules and their partners identified from proteomic data.Molecular & cellular proteomics : MCP · 2010Article
- A comprehensive molecular interaction map of the budding yeast cell cycle.Molecular systems biology · 2010Review
- Cyclin regulation by the s phase checkpoint.The Journal of biological chemistry · 2010Article
- An overview of Cdk1-controlled targets and processes.Cell division · 2010Article
- Perspectives on the DNA damage and replication checkpoint responses in Saccharomyces cerevisiae.DNA repair · 2009Review
- Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Rad53 of Saccharomyces cerevisiae is a checkpoint kinase whose structure and function are conserved among eukaryotes. When a cell detects damaged DNA, Rad53 activity is dramatically increased, which ultimately leads to changes in DNA replication, repair, and cell division. Despite its central role in checkpoint signaling, little is known about Rad53 substrates or substrate specificity. A number of proteins are implicated as Rad53 substrates; however, the evidence remains indirect. Previously, we have provided evidence that Swi6, a subunit of the Swi4/Swi6 late-G(1)-specific transcriptional activator, is a substrate of Rad53 in the G(1)/S DNA damage checkpoint. In the present study we identify Rad53 phosphorylation sites in Swi6 in vitro and demonstrate that at least one of them is targeted by Rad53 in vivo. Mutations in these phosphorylation sites in Swi6 shorten but do not eliminate the Rad53-dependent delay of the G(1)-to-S transition after DNA damage. We derive a consensus for Rad53 site preference at positions -2 and +2 (-2/+2) and identify its potential substrates in the yeast proteome. Finally, we present evidence that one of these candidates, the cohesin complex subunit Scc1 undergoes DNA damage-dependent phosphorylation, which is in part dependent on Rad53.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.