ArticlePlanta2003
Suborganellar localisation and effect of light on Helianthus tuberosus chloroplast transglutaminases and their substrates.
Article in Planta, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 64 citations in OpenAlex.
- At the core of global bioenergy: polyamines and transglutaminases in chloroplasts.Amino acids · 2026Review
- Programmed Cell Death Reversal: Polyamines, Effectors of the U-Turn from the Program of Death inInternational journal of molecular sciences · 2024Review
- Review of the Mechanisms by Which Transcription Factors and Exogenous Substances Regulate ROS Metabolism under Abiotic Stress.Antioxidants (Basel, Switzerland) · 2022Review
- Review
- Exogenous application of spermine and putrescine mitigate adversities of drought stress in wheat by protecting membranes and chloroplast ultra-structure.Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Overexpression of Transglutaminase from Cucumber in Tobacco Increases Salt Tolerance through Regulation of Photosynthesis.International journal of molecular sciences · 2019Article
- Polyamines - A New Metabolic Switch: Crosstalk With Networks Involving Senescence, Crop Improvement, and Mammalian Cancer Therapy.Frontiers in plant science · 2019Article
- Spermine Differentially Refines Plant Defense Responses Against Biotic and Abiotic Stresses.Frontiers in plant science · 2019Review
- TGase positively regulates photosynthesis via activation of Calvin cycle enzymes in tomato.Horticulture research · 2019Article
- Comparative transcriptome analysis of Zea mays in response to petroleum hydrocarbon stress.Environmental science and pollution research international · 2018Article
- Polyamine catabolism adds fuel to leaf senescence.Amino acids · 2017Review
- Dark-induced senescence of barley leaves involves activation of plastid transglutaminases.Amino acids · 2015Article
- Senescence and programmed cell death in plants: polyamine action mediated by transglutaminase.Frontiers in plant science · 2014Review
- Transglutaminases and their substrates in kinetin-stimulated etioplast-to-chloroplast transformation in cucumber cotyledons.Protoplasma · 2008Article
- Transglutaminases: widespread cross-linking enzymes in plants.Annals of botany · 2008Article
- The acropetal wave of developmental cell death of tobacco corolla is preceded by activation of transglutaminase in different cell compartments.Plant physiology · 2007Article
- Article
- AtPng1p. The first plant transglutaminase.Plant physiology · 2004Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The light stimulation of transglutaminase (TGase EC 2.3.2.13) activity was verified by incubating isolated chloroplasts of Helianthus tuberosus L. continuously or for alternate periods of light or dark (light/dark and dark/light). The first 10 min of incubation always represented the critical period. Light-harvesting complexes of photosystem II (LHCII) were more intensely labelled by (14)C-polyamines under light and light/dark than under dark and dark/light conditions. Chloroplasts were fractionated into thylakoid- and stroma-enriched fractions in which multiple TGase forms and substrates were found. Antibodies against TGase recognised 58- and 24-kDa bands in thylakoids and a 150-kDa band in the stroma. The latter, and its 150-kDa fraction, catalysed the conjugation of 14C-polyamines to Rubisco. In both fractions (thylakoid-pre and stroma-pre) the analysis of polyamine glutamyl derivatives showed a significant light-affected conjugation of polyamines to endogenous proteins. Alternatively, entire chloroplasts were incubated and afterwards their sub-fractions were isolated (thylakoid-post and stroma-post). The PSII and LHCII complexes were more intensely immunodetected in thylakoid-post than in thylakoid-pre, especially under dark conditions. Conversely, the conjugation of polyamines to thylakoid proteins was clearly light-stimulated in thylakoid-post, and much less in thylakoid-pre. Stroma-pre proteins were poorly polyamine-conjugated and not light-affected; on the contrary, stroma-post proteins were much more polyamine-modified and strongly light-stimulated. Thus, the light-activated conjugation depends mainly on the presence of the thylakoid fraction during the assay. The protective effect on chloroplasts under photo-damage, stress or senescence conditions attributed in the literature to free polyamines is discussed with regard to the occurrence of polyamine conjugates catalysed by TGases.
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