ArticleThe Biochemical journal2003
Evidence that TRPC1 (transient receptor potential canonical 1) forms a Ca(2+)-permeable channel linked to the regulation of cell volume in liver cells obtained using small interfering RNA targeted against TRPC1.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 80 citations in OpenAlex.
- Mechanobiological landscape of muscle stem cells.Skeletal muscle · 2026Review
- Central Osmolality Sensing for Arginine Vasopressin Release Is Mediated by WNK1-OSR1/SPAK-Kv3.1 Cascade.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Mechanosensitive ion channels in glaucoma pathophysiology.Vision research · 2024Review
- Mechanosensitive ion channel gene survey suggests potential roles in primary open angle glaucoma.Scientific reports · 2023Article
- Role of TRP Channels in Liver-Related Diseases.International journal of molecular sciences · 2023Review
- WNK1 promotes water homeostasis by acting as a central osmolality sensor for arginine vasopressin release.The Journal of clinical investigation · 2023Article
- Mechanosensitive ion channels in cell migration.Cells & development · 2021Review
- TRPM7 is an essential regulator for volume-sensitive outwardly rectifying anion channel.Communications biology · 2021Article
- Hypo-Osmotic Loading Induces Expression of IL-6 in Nucleus Pulposus Cells of the Intervertebral Disc Independent of TRPV4 and TRPM7.Frontiers in pharmacology · 2020Article
- Maitotoxin Is a Potential Selective Activator of the Endogenous Transient Receptor Potential Canonical Type 1 Channel in Xenopus laevis Oocytes.Marine drugs · 2017Article
- Hepatitis B virus modulates store-operated calcium entry to enhance viral replication in primary hepatocytes.PloS one · 2017Article
- Different Contribution of Redox-Sensitive Transient Receptor Potential Channels to Acetaminophen-Induced Death of Human Hepatoma Cell Line.Frontiers in pharmacology · 2016Article
- Interleukin-1 loop model for pathogenesis of Langerhans cell histiocytosis.Cell communication and signaling : CCS · 2015Review
- TRP channels and STIM/ORAI proteins: sensors and effectors of cancer and stroma cell migration.British journal of pharmacology · 2014Review
- Trpc2 depletion protects red blood cells from oxidative stress-induced hemolysis.Experimental hematology · 2012Article
- The opening of maitotoxin-sensitive calcium channels induces the acrosome reaction in human spermatozoa: differences from the zona pellucida.Asian journal of andrology · 2011Article
- Measuring Ca2+ influxes of TRPC1-dependent Ca2+ channels in HL-7702 cells with non-invasive micro-test technique.World journal of gastroenterology · 2009Article
- Physiology and pathophysiology of canonical transient receptor potential channels.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Review
- Hydrogen peroxide mobilizes Ca2+ through two distinct mechanisms in rat hepatocytes.Acta pharmacologica Sinica · 2009Article
- Dependence of regulatory volume decrease on transient receptor potential vanilloid 4 (TRPV4) expression in human corneal epithelial cells.Cell calcium · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The TRPC1 (transient receptor potential canonical 1) protein, which is thought to encode a non-selective cation channel activated by store depletion and/or an intracellular messenger, is expressed in a number of non-excitable cells. However, the physiological functions of TRPC1 are not well understood. The aim of these studies was to investigate the function of TRPC1 in liver cells using small interfering RNA (siRNA) to ablate the TRPC1 protein. Treatment of H4-IIE liver cells with siRNA targeted against TRPC1 caused an approx. 50% decrease in expression of the human TRPC1 protein in cells transfected with cDNA encoding human TRPC1, and a 50% decrease in expression of the endogenous TRPC1 protein (assessed by Western blot and immunofluorescence). The decrease in endogenous TRPC1 protein in cells transfected with TRPC1 siRNA was associated with a greater increase in cell volume (compared with the increase observed in control cells) immediately after cells were placed in a hypotonic medium, and an enhanced regulatory cell volume decrease after exposure to hypotonic medium. Treatment with siRNA targeted against TRPC1 also led to a 25% inhibition of thapsigargin-stimulated Ca(2+) inflow, a 40% inhibition of ATP and maitotoxin-stimulated Ca(2+) inflow, and a 50% inhibition of maitotoxin-stimulated Mn(2+) inflow. The idea that, in liver cells, TRPC1 encodes a non-selective cation channel involved directly or indirectly in the regulation of cell volume is consistent with the results obtained.
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