ArticleVirchows Archiv : an international journal of pathology2003
Vinculin: a novel marker for quiescent and activated hepatic stellate cells in human and rat livers.
Article in Virchows Archiv : an international journal of pathology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- The vinculin-β-catenin axis promotes bone formation and repair: an essential prerequisite for the anti-osteoporotic efficacy of sclerostin-neutralizing antibody.Science China. Life sciences · 2026Article
- Characterization of focal adhesion proteins in rodent hepatic stellate cells.Histochemistry and cell biology · 2022Article
- Hepatic stellate cells derived from the nestin-positive cells in septum transversum during rat liver development.Medical molecular morphology · 2018Article
- Ethanol targets nucleoredoxin/dishevelled interactions and stimulates phosphatidylinositol 4-phosphate production in vivo and in vitro.Biochemical pharmacology · 2018Article
- Identification of markers for quiescent pancreatic stellate cells in the normal human pancreas.Histochemistry and cell biology · 2017Article
- Signaling of extracellular matrices for tissue regeneration and therapeutics.Tissue engineering and regenerative medicine · 2016Review
- All-In-One: Advanced preparation of Human Parenchymal and Non-Parenchymal Liver Cells.PloS one · 2015Article
- Cytoglobin is expressed in hepatic stellate cells, but not in myofibroblasts, in normal and fibrotic human liver.Laboratory investigation; a journal of technical methods and pathology · 2014Article
- Antifibrotic properties of transarterial oncolytic VSV therapy for hepatocellular carcinoma in rats with thioacetamide-induced liver fibrosis.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Blebbistatin inhibits contraction and accelerates migration in mouse hepatic stellate cells.British journal of pharmacology · 2010Article
- Vinculin and cellular retinol-binding protein-1 are markers for quiescent and activated hepatic stellate cells in formalin-fixed paraffin embedded human liver.Histochemistry and cell biology · 2009Article
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
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Abstract
In liver injuries, the quiescent hepatic stellate cells (HSCs) promptly change to activated HSCs, which are easily identified by the intense immunoreactivity for alpha-smooth muscle actin. However, reproducible markers for quiescent HSCs in formalin-fixed, paraffin-embedded liver tissue sections have not yet been reported. We immunohistochemically examined the expression of vinculin, one major protein of dense plaques, on cultured LI90 cells and on HSCs in normal and diseased human and rat livers. In cultured LI90 cells, vinculin appeared as small linear patches. Although vinculin was consistently negative in the routine liver tissue sections, an antigen retrieval technique using microwave oven heating yielded excellent effects. Using this technique, the formalin-fixed, paraffin-embedded human and rat normal liver tissue sections showed the vinculin immunoreactivity along the sinusoidal wall. Immunoelectron microscopic observation of hepatic parenchyma demonstrated that the vinculin was exclusively expressed in quiescent HSCs. In fetal rat livers, vinculin-positive quiescent HSCs gradually increased in number with gestation. In diseased livers the activated HSCs showed more intense immunoreaction for vinculin. These results indicate that, using microwave pretreatment, vinculin is expressed in quiescent and activated HSCs in routinely processed liver tissue sections. It could allow us to evaluate the development and distribution of quiescent HSCs and to examine the relationship between quiescent and activated HSCs.
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