ArticleJournal of virology2003
In situ characterization of Cymbidium Ringspot Tombusvirus infection-induced posttranscriptional gene silencing in Nicotiana benthamiana.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 101 citations in OpenAlex.
- Three decades of mobile RNA silencing within plants: what have we learnt?Journal of experimental botany · 2026Review
- Small RNA mobility and plant virus diseases.Journal of experimental botany · 2026Review
- The plant siRNA landscape.The Plant cell · 2024Review
- Salicylic acid and RNA interference mediate antiviral immunity of plant stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Infection Defects of RNA and DNA Viruses Induced by Antiviral RNA Interference.Microbiology and molecular biology reviews : MMBR · 2023Review
- Roles of RNA silencing in viral and non-viral plant immunity and in the crosstalk between disease resistance systems.Nature reviews. Molecular cell biology · 2022Review
- The R2R3-MYB transcription factor FaMYB63 participates in regulation of eugenol production in strawberry.Plant physiology · 2022Article
- Atypical molecular features of RNA silencing against the phloem-restricted polerovirus TuYV.Nucleic acids research · 2021Article
- Transcriptome reprogramming in the shoot apical meristem of CymRSV-infected Nicotiana benthamiana plants associates with viral exclusion and the lack of recovery.Molecular plant pathology · 2019Article
- Article
- Distinct Effects of p19 RNA Silencing Suppressor on Small RNA Mediated Pathways in Plants.PLoS pathogens · 2016Article
- Small RNAs break out: the molecular cell biology of mobile small RNAs.Nature reviews. Molecular cell biology · 2014Review
- The protein kinase TOUSLED facilitates RNAi in Arabidopsis.Nucleic acids research · 2014Article
- RNA silencing suppression by plant pathogens: defence, counter-defence and counter-counter-defence.Nature reviews. Microbiology · 2013Review
- Protein body formation in stable transgenic tobacco expressing elastin-like polypeptide and hydrophobin fusion proteins.BMC biotechnology · 2013Article
- Viral and cellular factors involved in Phloem transport of plant viruses.Frontiers in plant science · 2013Article
- Article
- Ectopic expression of the p23 silencing suppressor of Citrus tristeza virus differentially modifies viral accumulation and tropism in two transgenic woody hosts.Molecular plant pathology · 2011Article
- Phytobacterial type III effectors HopX1, HopAB1 and HopF2 enhance sense-post-transcriptional gene silencing independently of plant R gene-effector recognition.Molecular plant-microbe interactions : MPMI · 2011Article
- The specific binding to 21-nt double-stranded RNAs is crucial for the anti-silencing activity of Cucumber vein yellowing virus P1b and perturbs endogenous small RNA populations.RNA (New York, N.Y.) · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants, posttranscriptional gene silencing (PTGS) is an ancient and effective defense mechanism against viral infection. A number of viruses encode proteins that suppress virus-activated PTGS. The p19 protein of tombusviruses is a potent PTGS suppressor which interferes with the onset of PTGS-generated systemic signaling and is not required for viral replication or for viral movement in Nicotiana benthamiana. This unique feature of p19 suppressor allowed us to analyze the mechanism of PTGS-based host defense and its viral suppression without interfering with other viral functions. In contrast to the necrotic symptoms caused by wild-type tombusvirus, the infection of p19-defective mutant virus results in the development of a typical PTGS-associated recovery phenotype in N. benthamiana. In this report we show the effect of PTGS on the viral infection process for N. benthamiana infected with either wild-type Cymbidium Ringspot Tombusvirus (CymRSV) or a p19-defective mutant (Cym19stop). In situ analyses of different virus-derived products revealed that PTGS is not able to reduce accumulation of virus in primary infected cells regardless of the presence of p19 PTGS suppressor. We also showed that both CymRSV and Cym19stop viruses move systemically in the vasculature, with similar efficiencies. However, in contrast to the uniform accumulation of CymRSV throughout systemically infected leaves, the presence of Cym19stop virus was confined to and around the vascular bundles. These results suggest that the role of p19 is to prevent the onset of mobile signal-induced systemic PTGS ahead of the viral infection front, leading to generalized infection.
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