Evidence map›Paper›PMID 12719600›Full record

ArticleJournal of virology2003

Green fluorescent protein-tagged retroviral envelope protein for analysis of virus-cell interactions.

Dirk Spitzer, Kurt E J Dittmar, Manfred Rohde, Hansjörg Hauser, Dagmar Wirth

Open access · greenAbstract readEvaluation Study
In one paragraph

Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 15 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Dirk SpitzerDepartment of Gene Regulation and Differentiation, German Research Center for Biotechnology, D-38124 Braunschweig, Germany.
Kurt E J Dittmar
Manfred Rohde
Hansjörg Hauser
Dagmar Wirth
German Center for Infection Research · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluorescent retroviral envelope (Env) proteins were developed for direct visualization of viral particles. By fusing the enhanced green fluorescent protein (eGFP) to the N terminus of the amphotropic 4070A envelope protein, extracellular presentation of eGFP was achieved. Viruses incorporated the modified Env protein and efficiently infected cells. We used the GFP-tagged viruses for staining retrovirus receptor-positive cells, thereby circumventing indirect labeling techniques. By generating cells which conditionally expressed the GFP-tagged Env protein, we could confirm an inverse correlation between retroviral Env expression and infectivity (superinfection). eGFP-tagged virus particles are suitable for monitoring the dynamics of virus-cell interactions.

Indexed as

3T3 CellsAnimalsGreen Fluorescent ProteinsLuminescent ProteinsMiceMicroscopy, ConfocalReceptors, VirusRecombinant Fusion ProteinsRetroviridaeTransduction, GeneticViral Envelope ProteinsGreen Fluorescent ProteinsLuminescent ProteinsReceptors, VirusRecombinant Fusion ProteinsViral Envelope Proteins

Identifiers

PMID12719600
PMCPMC154001
OpenAlexW2134612137

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.