ArticleProceedings of the National Academy of Sciences of the United States of America2003
Identification of genes involved in cell invasion by using a library of randomized hybrid ribozymes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 51 citations in OpenAlex.
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- A KrasG12D-driven genetic mouse model of pancreatic cancer requires glypican-1 for efficient proliferation and angiogenesis.Oncogene · 2012Article
- Isolation and molecular characterization of Rem2 isoforms in the rainbow trout (Oncorhynchus mykiss): Tissue and central nervous system expression.Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology · 2012Article
- Parkin enhances the expression of cyclin-dependent kinase 6 and negatively regulates the proliferation of breast cancer cells.The Journal of biological chemistry · 2010Article
- ALDH isozymes downregulation affects cell growth, cell motility and gene expression in lung cancer cells.Molecular cancer · 2008Article
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- The RGK family of GTP-binding proteins: regulators of voltage-dependent calcium channels and cytoskeleton remodeling.Cellular signalling · 2008Review
- Loss-of-function screening by randomized intracellular antibodies: identification of hnRNP-K as a potential target for metastasis.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- A universal plasmid library encoding all permutations of small interfering RNA.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Identification of cellular cofactors for human immunodeficiency virus replication via a ribozyme-based genomics approach.Journal of virology · 2004Article
- Induction and characterization of taxol-resistance phenotypes with a transiently expressed artificial transcriptional activator library.Nucleic acids research · 2004Article
- An RNA-dependent protein kinase is involved in tunicamycin-induced apoptosis and Alzheimer's disease.The EMBO journal · 2004Article
- Surfection: a new platform for transfected cell arrays.Nucleic acids research · 2004Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Libraries of RNA helicase-coupled randomized ribozymes are a powerful tool for the identification of functional genes. We have demonstrated the usefulness of this functional gene-discovery system by identifying genes involved in tumor invasion, a process that is an essential feature of tumor metastasis: the spread of cancer cells from the original tumor to other sites in the body that imposes serious problems in the prognosis and treatment of cancer. Using a filter-based invasion assay in vitro, we isolated ribozymes that enhanced the invasive properties of NIH 3T3 fibroblasts. Sequence analysis of selected clones and a database search revealed that genes such as the gene for Gem GTPase and uncharacterized genes that resemble genes for myosin phosphatase and protein-tyrosine-phosphatase are involved in cell invasion. Our system for gene identification by using ribozymes and the functional analysis of target genes should help to clarify the complex mechanisms of invasion and metastasis and might provide information that is relevant to cancer therapy.
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