ArticleThe Journal of cell biology2003
LST8 negatively regulates amino acid biosynthesis as a component of the TOR pathway.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed, 142 citations in OpenAlex.
- Target of Rapamycin is a crucial regulator of photosynthesis and nutrient metabolism partitioning in Nannochloropsis gaditana.Biotechnology for biofuels and bioproducts · 2025Article
- NgLst8 Coactivates TOR Signaling to Activate Photosynthetic Growth inMicroorganisms · 2024Article
- Assembly of mTORC3 Involves Binding of ETV7 to Two Separate Sequences in the mTOR Kinase Domain.International journal of molecular sciences · 2024Article
- The allosteric mechanism of mTOR activation can inform bitopic inhibitor optimization.Chemical science · 2024Article
- Transcriptome Analysis of the Influence of High-Pressure Carbon Dioxide onJournal of fungi (Basel, Switzerland) · 2022Article
- Stress- and metabolic responses of Candida albicans require Tor1 kinase N-terminal HEAT repeats.PLoS pathogens · 2022Article
- Mitochondrial-derived compartments facilitate cellular adaptation to amino acid stress.Molecular cell · 2021Article
- HJournal of fungi (Basel, Switzerland) · 2021Article
- Chemical-Genetic Interactions with the Proline Analog L-Azetidine-2-Carboxylic Acid inG3 (Bethesda, Md.) · 2020Article
- The Ccr4-Not complex regulates TORC1 signaling and mitochondrial metabolism by promoting vacuole V-ATPase activity.PLoS genetics · 2020Article
- MELK promotes Endometrial carcinoma progression via activating mTOR signaling pathway.EBioMedicine · 2020Article
- Nutrient Signaling and Lysosome Positioning Crosstalk Through a Multifunctional Protein, Folliculin.Frontiers in cell and developmental biology · 2020Review
- Genome Wide Analysis of WD40 Proteins in Saccharomyces cerevisiae and Their Orthologs in Candida albicans.The protein journal · 2019Article
- Feedback regulation of TORC1 by its downstream effectors Npr1 and Par32.Molecular biology of the cell · 2018Article
- Signal integration in the (m)TORC1 growth pathway.Frontiers in biology · 2018Article
- Regulation of Sensing, Transportation, and Catabolism of Nitrogen Sources in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2018Review
- The TOR Signaling Pathway in Spatial and Temporal Control of Cell Size and Growth.Frontiers in cell and developmental biology · 2017Review
- Tor forms a dimer through an N-terminal helical solenoid with a complex topology.Nature communications · 2016Article
- Role of the mammalian target of rapamycin pathway in lentiviral vector transduction of hematopoietic stem cells.Current opinion in hematology · 2015Review
- La-related Protein 1 (LARP1) Represses Terminal Oligopyrimidine (TOP) mRNA Translation Downstream of mTOR Complex 1 (mTORC1).The Journal of biological chemistry · 2015Article
8 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
LST8, a Saccharomyces cerevisiae gene encoding a 34-kD WD-repeat protein, was identified by mutations that caused defects in sorting Gap1p to the plasma membrane. Here, we report that the Gap1p sorting defect in the lst8-1 mutant results from derepression of Rtg1/3p activity and the subsequent accumulation of high levels of intracellular amino acids, which signal Gap1p sorting to the vacuole. To identify the essential function of Lst8p, we isolated lst8 mutants that are temperature-sensitive for growth. These mutants show hypersensitivity to rapamycin and derepressed Gln3p activity like cells with compromised TOR pathway activity. Like tor2 mutants, lst8 mutants also have cell wall integrity defects. Confirming a role for Lst8p in the TOR pathway, we find that Lst8p associates with both Tor1p and Tor2p and is a peripheral membrane protein that localizes to endosomal or Golgi membranes and cofractionates with Tor1p. Further, we show that a sublethal concentration of rapamycin mimics the Gap1p sorting defect of an lst8 mutant. Finally, the different effects of lst8 alleles on the activation of either the Rtg1/3p or Gln3p transcription factors reveal that these two pathways constitute distinct, genetically separable outputs of the Tor-Lst8 regulatory complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.