ArticleThe Journal of cell biology2003
Sim4: a novel fission yeast kinetochore protein required for centromeric silencing and chromosome segregation.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
70 citing papers in PubMed, 109 citations in OpenAlex.
- Inositol Pyrophosphate-Controlled Kinetochore Architecture and Mitotic Entry inJournal of fungi (Basel, Switzerland) · 2022Article
- The Mis6 inner kinetochore subcomplex maintains CENP-A nucleosomes against centromeric non-coding transcription during mitosis.Communications biology · 2022Article
- Perturbation of kinetochore function using GFP-binding protein in fission yeast.G3 (Bethesda, Md.) · 2021Article
- Conservation of centromeric histone 3 interaction partners in plants.Journal of experimental botany · 2020Article
- Hap2-Ino80-facilitated transcription promotes de novo establishment of CENP-A chromatin.Genes & development · 2020Article
- Negative Regulation of the Mis17-Mis6 Centromere Complex by mRNA Decay Pathway and EKC/KEOPS Complex inG3 (Bethesda, Md.) · 2019Article
- Intricate regulation on epigenetic stability of the subtelomeric heterochromatin and the centromeric chromatin in fission yeast.Current genetics · 2019Review
- Centromere DNA Destabilizes H3 Nucleosomes to Promote CENP-A Deposition during the Cell Cycle.Current biology : CB · 2018Article
- Ccp1 modulates epigenetic stability at centromeres and affects heterochromatin distribution inThe Journal of biological chemistry · 2018Article
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- The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.The Journal of cell biology · 2017Article
- Kinetochore Components Required for Centromeric Chromatin Assembly Are Impacted by Msc1 inGenetics · 2017Article
- The Ino80 complex mediates epigenetic centromere propagation via active removal of histone H3.Nature communications · 2017Article
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- Epigenetic Regulation of Chromatin States in Schizosaccharomyces pombe.Cold Spring Harbor perspectives in biology · 2015Review
- The CENP-T C-terminus is exclusively proximal to H3.1 and not to H3.2 or H3.3.International journal of molecular sciences · 2015Article
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- Article
- Centromeric barrier disruption leads to mitotic defects in Schizosaccharomyces pombe.G3 (Bethesda, Md.) · 2014Article
- The RFTS domain of Raf2 is required for Cul4 interaction and heterochromatin integrity in fission yeast.PloS one · 2014Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Fission yeast centromeres are composed of two domains: the central core and the outer repeats. Although both regions are required for full centromere function, the central core has a distinct chromatin structure and is likely to underlie the kinetochore itself, as it is associated with centromere-specific proteins. Genes placed within either region are transcriptionally silenced, reflecting the formation of a functional kinetochore complex and flanking centromeric heterochromatin. Here, transcriptional silencing was exploited to identify components involved in central core silencing and kinetochore assembly or structure. The resulting sim (silencing in the middle of the centromere) mutants display severe chromosome segregation defects. sim2+ encodes a known kinetochore protein, the centromere-specific histone H3 variant Cnp1CENP-A. sim4+ encodes a novel essential coiled-coil protein, which is specifically associated with the central core region and is required for the unusual chromatin structure of this region. Sim4 coimmunoprecipitates with the central core component Mis6 and, like Mis6, affects Cnp1CENP-A association with the central domain. Functional Mis6 is required for Sim4 localization at the kinetochore. Our analyses illustrate the fundamental link between silencing, chromatin structure, and kinetochore function, and establish defective silencing as a powerful approach for identifying proteins required to build a functional kinetochore.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.