ArticleBiophysical journal2003
Rationalization of membrane protein crystallization with polyethylene glycol using a simple depletion model.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- A note on the appearance of PEG in macromolecular crystals.Acta crystallographica. Section F, Structural biology communications · 2026Article
- Towards understanding the crystallization of photosystem II: influence of poly(ethylene glycol) of various molecular sizes on the micelle formation of alkyl maltosides.Photosynthesis research · 2024Article
- Defined PEG smears as an alternative approach to enhance the search for crystallization conditions and crystal-quality improvement in reduced screens.Acta crystallographica. Section D, Biological crystallography · 2015Article
- PEG-protein interaction induced contraction of NalD chains.PloS one · 2014Article
- Selecting temperature for protein crystallization screens using the temperature dependence of the second virial coefficient.PloS one · 2011Article
- Development of an ELISA method for detecting immune complexes between tissue-nonspecific alkaline phosphatase and immunoglobulin G.Journal of clinical laboratory analysis · 2007Article
- Membrane protein crystallization in amphiphile phases: practical and theoretical considerations.Progress in biophysics and molecular biology · 2005Review
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4 authors.
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Abstract
Based on the importance of crystallizing membrane proteins in a rational way, cytochrome bc(1) complex (BC1) was crystallized using polyethylene glycol (PEG) as a sole crystallization agent. Interaction between protein-detergent complexes of BC1 was estimated by dynamic light scattering, and was compared with the numerical calculation using the Derjaguin-Landau-Verwey-Overbeek potential plus a depletion potential, without considering specific surface properties of the protein-detergent complexes. The experiments and calculation were found to be consistent and we obtained a relation between PEG molecular weight M and the range of depletion zone delta as delta approximately M(0.48+/-0.02). The stability of liquid phase of BC1 solutions was controlled by a ratio of (the range of depletion zone)/(the radius of a BC1 particle), which was consistent with recent theoretical predictions. The crystallization was most successful under a condition where the stability of the liquid phase changed from stable to unstable. The PEG molecular weight that fulfilled this condition coincided with the one used empirically to crystallize BC1 in the past by a number of groups. These results are compared to the fact that membrane proteins were often successfully crystallized close to the detergent cloud point.
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