ArticleBiophysical journal2003
The photochemical reaction cycle of proteorhodopsin at low pH.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 61 citations in OpenAlex.
- Diverging pH dependence and photocycle dynamics across members of the CryoRhodopsin clade.Biophysical journal · 2026Article
- Microbial Rhodopsins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Electrostatic Environment of Proteorhodopsin Affects the pKa of Its Buried Primary Proton Acceptor.Biophysical journal · 2020Article
- Functionally Active Membrane Proteins Incorporated in Mesostructured Silica Films.Journal of the American Chemical Society · 2018Article
- Gloeobacter rhodopsin, limitation of proton pumping at high electrochemical load.Biophysical journal · 2013Article
- Lipid bilayer composition can influence the orientation of proteorhodopsin in artificial membranes.Biophysical journal · 2013Article
- The EF loop in green proteorhodopsin affects conformation and photocycle dynamics.Biophysical journal · 2013Article
- Characterization of the ground state dynamics of proteorhodopsin by NMR and optical spectroscopies.Journal of biomolecular NMR · 2012Article
- Green proteorhodopsin reconstituted into nanoscale phospholipid bilayers (nanodiscs) as photoactive monomers.Journal of the American Chemical Society · 2011Article
- Time-resolved WAXS reveals accelerated conformational changes in iodoretinal-substituted proteorhodopsin.Biophysical journal · 2011Article
- Two bathointermediates of the bacteriorhodopsin photocycle, from time-resolved nanosecond spectra in the visible.The journal of physical chemistry. B · 2009Article
- Raman spectroscopy reveals direct chromophore interactions in the Leu/Gln105 spectral tuning switch of proteorhodopsins.The journal of physical chemistry. B · 2008Article
- Initial reaction dynamics of proteorhodopsin observed by femtosecond infrared and visible spectroscopy.Biophysical journal · 2008Article
- Characterization of the primary photochemistry of proteorhodopsin with femtosecond spectroscopy.Biophysical journal · 2008Article
- First steps of retinal photoisomerization in proteorhodopsin.Biophysical journal · 2006Article
- The nitrate transporting photochemical reaction cycle of the pharaonis halorhodopsin.Biophysical journal · 2004Article
- pH-dependent transitions in xanthorhodopsin.Photochemistry and photobiologyArticle
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
Abstract
The proton acceptor group in the recently described retinal protein, proteorhodopsin has an unusually high pK(a) of 7.1. It was shown that at pH above this pK(a), illumination initiates a photocycle similar to that of bacteriorhodopsin, and the protein transports proton across the cell membrane. Recently it was reported that proteorhodopsin, unlike bacteriorhodopsin, transports protons at pH below the pK(a) of the proton acceptor, and this transport is in the reverse direction. We have investigated the photocycle of proteorhodopsin at such low pH. At pH 5, three spectrally distinct intermediates K, L, and N, and another spectrally silent one, PR', could be identified, but a deprotonated Schiff base containing M-like intermediate, characteristic for proton pumping activity, does not accumulate. All the reactions between the intermediates are close to equilibrium, except the last transition from PR' to PR, when the protein returns to its initial unexcited state in a quasiunidirectional reaction. The electric signal measurements indicate that although charge motions are detected inside the protein, their net dislocation is zero, indicating that contrary to the earlier reported, at low pH no charged particle is transported across the membrane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.