ArticleBiophysical journal2003
Cholesterol in bilayers of sphingomyelin or dihydrosphingomyelin at concentrations found in ocular lens membranes.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 73 citations in OpenAlex.
- Physical Properties of Lens Membranes in Animals with Different Lifespans.Biomolecules · 2025Article
- Per- and Polyfluoroalkyl Substances: Impacts on Morphology, Behavior and Lipid Levels in Zebrafish Embryos.Toxics · 2024Article
- Metabolomic Profiling in Patients with Different Hemodynamic Subtypes of Severe Aortic Valve Stenosis.Biomolecules · 2023Article
- Assembly formation of minor dihydrosphingomyelin in sphingomyelin-rich ordered membrane domains.Scientific reports · 2020Article
- Formation of cholesterol Bilayer Domains Precedes Formation of Cholesterol Crystals in Membranes Made of the Major Phospholipids of Human Eye Lens Fiber Cell Plasma Membranes.Current eye research · 2020Article
- Confocal Microscopy Confirmed that in Phosphatidylcholine Giant Unilamellar Vesicles with very High Cholesterol Content Pure Cholesterol Bilayer Domains Form.Cell biochemistry and biophysics · 2019Article
- Why Is Very High Cholesterol Content Beneficial for the Eye Lens but Negative for Other Organs?Nutrients · 2019Review
- Solvatochromic Modeling of Laurdan for Multiple Polarity Analysis of Dihydrosphingomyelin Bilayer.Biophysical journal · 2019Article
- Is the cholesterol bilayer domain a barrier to oxygen transport into the eye lens?Biochimica et biophysica acta. Biomembranes · 2018Article
- Cholesterol Bilayer Domains in the Eye Lens Health: A Review.Cell biochemistry and biophysics · 2017Review
- The Influence of Hydrogen Bonding on Sphingomyelin/Colipid Interactions in Bilayer Membranes.Biophysical journal · 2016Article
- Orientation and Order of the Amide Group of Sphingomyelin in Bilayers Determined by Solid-State NMR.Biophysical journal · 2015Article
- Properties of membranes derived from the total lipids extracted from clear and cataractous lenses of 61-70-year-old human donors.European biophysics journal : EBJ · 2015Article
- Early and late HIV-1 membrane fusion events are impaired by sphinganine lipidated peptides that target the fusion site.The Biochemical journal · 2014Article
- Formation of cholesterol bilayer domains precedes formation of cholesterol crystals in cholesterol/dimyristoylphosphatidylcholine membranes: EPR and DSC studies.The journal of physical chemistry. B · 2013Article
- Comparative computer simulation study of cholesterol in hydrated unary and binary lipid bilayers and in an anhydrous crystal.The journal of physical chemistry. B · 2013Article
- Sphingopeptides: dihydrosphingosine-based fusion inhibitors against wild-type and enfuvirtide-resistant HIV-1.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Saturation with cholesterol increases vertical order and smoothes the surface of the phosphatidylcholine bilayer: a molecular simulation study.Biochimica et biophysica acta · 2012Article
- Phases and domains in sphingomyelin-cholesterol membranes: structure and properties using EPR spin-labeling methods.European biophysics journal : EBJ · 2012Article
- Sphingomyelinase D activity in model membranes: structural effects of in situ generation of ceramide-1-phosphate.PloS one · 2012Article
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1 author at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Membranes of the lens of the eye of mammals have two particular characteristics, high concentrations of sphingomyelin, and dihydrosphingomyelin and cholesterol. We have studied the miscibility of cholesterol with both egg sphingomyelin and with dihydrosphingomyelin made by hydrogenation of egg sphingomyelin. At a cholesterol mol fraction of 0.5 and lower, crystallites of cholesterol are not present with either form of sphingomyelin, as observed by differential scanning calorimetry and by (13)C CP/MAS NMR. However, in the range of 0.6 to 0.8 mol fraction of cholesterol increasing amounts of crystallites form, with the amount of anhydrous cholesterol crystals formed being somewhat greater with dihyrosphingomyelin compared with sphingomyelin. Interestingly, cholesterol monohydrate crystallites formed in these two phospholipids exhibit a temperature of dehydration higher than that of pure cholesterol monohydrate crystals. These cholesterol monohydrate crystals form more rapidly and in greater amounts with the unmodified form of sphingomyelin. This difference is likely a consequence of differences at the membrane interface. The chemical shift of the (13)C of the carbonyl group, as measured by CP/MAS NMR, shows that there are differences between the two phospholipids in both the presence and absence of cholesterol. The bilayers with dihydrosphingomyelin are more hydrogen bonded. Cholesterol crystallites are known to be present in the lens of the eye. Our studies show that the ratio of sphingomyelin to dihydrosphingomyelin can affect the rate of formation of these cholesterol crystallites and thus play a role in the membrane of cells of the lens, affecting ocular function.
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