ArticleMolecular therapy : the journal of the American Society of Gene Therapy2003
Noninvasive imaging of lentiviral-mediated reporter gene expression in living mice.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 108 citations in OpenAlex.
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- Regression of experimental NIS-expressing breast cancer brain metastases in response to radioiodide/gemcitabine dual therapy.Oncotarget · 2016Article
- Visualization of the Biological Behavior of Tumor-Associated Macrophages in Living Mice with Colon Cancer Using Multimodal Optical Reporter Gene Imaging.Neoplasia (New York, N.Y.) · 2016Article
- Radiolabeling strategies for radionuclide imaging of stem cells.Stem cell reviews and reports · 2015Article
- A new multicolor bioluminescence imaging platform to investigate NF-κB activity and apoptosis in human breast cancer cells.PloS one · 2014Article
- Exogenous MC3T3 preosteoblasts migrate systemically and mitigate the adverse effects of wear particles.Tissue engineering. Part A · 2012Article
- Remnant living cells that escape cell loss in late-stage tumors exhibit cancer stem cell-like characteristics.Cell death & disease · 2012Article
- In vivo Noninvasive Small Animal Molecular Imaging.Osong public health and research perspectives · 2012Review
- Noninvasive cell-tracking methods.Nature reviews. Clinical oncology · 2011Review
- Noninvasive monitoring of placenta-specific transgene expression by bioluminescence imaging.PloS one · 2011Article
- Nanoparticles for cell labeling.Nanoscale · 2011Review
- Continuous infusion of UHMWPE particles induces increased bone macrophages and osteolysis.Clinical orthopaedics and related research · 2011Article
- Imaging of embryonic stem cell migration in vivo.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Surveillance of systemic trafficking of macrophages induced by UHMWPE particles in nude mice by noninvasive imaging.Journal of biomedical materials research. Part A · 2010Article
- The noninvasive, quantitative, in vivo assessment of adenoviral-mediated gene delivery in skin wound biomaterials.Biomaterials · 2009Article
- Noninvasive bioluminescent imaging demonstrates long-term multilineage engraftment of ex vivo-expanded CD34-selected umbilical cord blood cells.Stem cells (Dayton, Ohio) · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Lentiviral-mediated gene delivery holds significant promise for sustained gene expression within living systems. Vesicular stomatitis virus glycoprotein-pseudotyped human immunodeficiency virus type 1-based lentiviral vectors can be used to introduce transgenes in a broad spectrum of dividing as well as nondividing cells. In the current study, we construct a lentiviral vector carrying two reporter genes separated by an internal ribosomal entry site and utilize that virus in delivering both genes into neuroblastoma cells in cell culture and into cells implanted in living mice. We utilize two reporter genes, a mutant herpes simplex virus type 1 (HSV1) sr39tk as a reporter gene compatible with positron emission tomography (PET) and a bioluminescent optical reporter gene, firefly luciferase (Fluc), to image expression in living mice by an optical charge-coupled device (CCD) camera. By using this lentivirus, neuroblastoma (N2a) cells are stably transfected and a high correlation (R(2) = 0.91) between expressions of the two reporter genes in cell culture is established. Imaging of both reporter genes using microPET and optical CCD camera in living mice is feasible, with the optical approach being more sensitive, and a high correlation (R(2) = 0.86) between gene expressions is again observed in lentiviral-infected N2a tumor xenografts. Indirect imaging of HSV1-sr39tk suicide gene therapy utilizing Fluc is also feasible and can be detected with increased sensitivity by using the optical CCD. These preliminary results validate the use of lentiviral vectors carrying reporter genes for multimodality imaging of gene expression and should have many applications, including imaging of xenografts, metastasis, and cell trafficking as well as noninvasive monitoring of lentiviral-mediated gene delivery and expression.
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Registered trials
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