ArticleThe Biochemical journal2003
Heterologous expression of four glutathione transferase genes genetically linked to a major insecticide-resistance locus from the malaria vector Anopheles gambiae.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers, 1 of them a synthesis that pooled it.
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Who cites it
64 citing papers in PubMed, 1 synthesis or guideline pooled it, 219 citations in OpenAlex.
- A multi-omic meta-analysis reveals novel mechanisms of insecticide resistance in malaria vectors.Communications biology · 2025Pooled it
- Multi-omics analysis identifies loci associated with pyrethroid resistance across sister species in the Anopheles gambiae species complex.BMC genomics · 2026Article
- Molecular and Biochemical Characterization of Insecticide Resistance in Field-CaughtACS omega · 2026Article
- Insecticide resistance status and mechanisms in Aedes aegypti and Aedes albopictus from different dengue endemic regions of Panama.Tropical medicine and health · 2024Article
- Uncovering the genetic diversity in Aedes aegypti insecticide resistance genes through global comparative genomics.Scientific reports · 2024Article
- Potent GST Ketosteroid Isomerase Activity Relevant to Ecdysteroidogenesis in the Malaria VectorBiomolecules · 2023Article
- Role of Insect and Mammal Glutathione Transferases in Chemoperception.Biomolecules · 2023Review
- Plant-Based Bioinsecticides for Mosquito Control: Impact on Insecticide Resistance and Disease Transmission.Insects · 2022Review
- Functional and Structural Diversity of Insect Glutathione S-transferases in Xenobiotic Adaptation.International journal of biological sciences · 2022Review
- Transcriptome Analysis of Detoxification-Related Genes in Spodoptera frugiperda (Lepidoptera: Noctuidae).Journal of insect science (Online) · 2022Article
- Potentiation Effects of Ficus sycomorus Active Fraction Against Permethrin-Resistant Field-Population of Anopheles coluzzii (Diptera: Culicidae).Neotropical entomology · 2021Article
- Cloning and expression studies on glutathione S-transferase like-gene in honey bee for its role in oxidative stress.Cell stress & chaperones · 2021Article
- Investigation of DDT resistance mechanisms in Anopheles funestus populations from northern and southern Benin reveals a key role of the GSTe2 gene.Malaria journal · 2020Article
- The genome of pest Rhynchophorus ferrugineus reveals gene families important at the plant-beetle interface.Communications biology · 2020Article
- Biological Impact and Enzyme Activities ofFrontiers in physiology · 2020Article
- Functional genetic validation of key genes conferring insecticide resistance in the major African malaria vector,Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Characterisation of Anopheles strains used for laboratory screening of new vector control products.Parasites & vectors · 2019Article
- Transcriptome Sequencing and Analysis of Changes Associated with Insecticide Resistance in the Dengue Mosquito (The American journal of tropical medicine and hygiene · 2019Article
- Molecular characterization of carbendazim resistance of Fusarium species complex that causes sugarcane pokkah boeng disease.BMC genomics · 2019Article
- Insecticidal Activities of Chloramphenicol Derivatives Isolated from a Marine Alga-Derived Endophytic Fungus,Molecules (Basel, Switzerland) · 2018Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cluster of eight genes encoding glutathione transferases (GSTs) are located on division 33B of polytene chromosome arm 3R of the African malaria mosquito, Anopheles gambiae. This region of the genome contains a major 1,1,1-trichloro-2,2-bis-( p -chlorophenyl)ethane (DDT)-resistance locus, rtd1. These GSTs belong to the insect-specific Epsilon class and share between 22.6 and 65.2% identity at the amino acid level. Two distinct allelic variants of the Epsilon GST, GSTe1, differing at 12 out of 224 amino acid residues, are present in laboratory and field populations of A. gambiae. To investigate the possible role of these GSTs in conferring resistance to the insecticide DDT, both GSTe1 alleles, plus three additional members of this gene cluster, were expressed in Escherichia coli and the recombinant proteins biochemically characterized. The five putative glutathione transferases encoded catalytically active subunits with variable biochemical properties. For example, the two allelic variants of GSTE1-1 encoded proteins with over 100-fold variation in peroxidase activity, while the three remaining GSTs had no detectable peroxidase activity. Only GSTE2-2 was able to metabolize DDT. Western blots using antibodies raised against these GSTs indicated that the expression of GSTE2-2 is elevated in a DDT-resistant strain of A. gambiae.
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