ArticleWorld journal of gastroenterology2003
Laminin induces the expression of cytokeratin 19 in hepatocellular carcinoma cells growing in culture.
Article in World journal of gastroenterology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed, 18 citations in OpenAlex.
- 3D bioprinting of hepatocytes: core-shell structured co-cultures with fibroblasts for enhanced functionality.Scientific reports · 2021Article
- Progenitor-derived hepatocellular carcinoma model in the rat.Hepatology (Baltimore, Md.) · 2010Article
- Is there a place for serum laminin determination in patients with liver disease and cancer?World journal of gastroenterology · 2008Review
- Serum CYFRA 21-1 level reflects hepatocellular carcinoma metastasis: study in nude mice model and clinical patients.Journal of cancer research and clinical oncology · 2006Article
- Detection of micrometastasis of gastric carcinoma in peripheral blood circulation.World journal of gastroenterology · 2004Article
- Rapid and high throughput detection of HBV YMDD mutants with fluorescence polarization.World journal of gastroenterology · 2003Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo study the abnormal cytokeratin (CK) expression, emergence of CK19 with or without CK7, in liver parenchymal cells and the role of laminin (LN), a basement membrane protein, in this process.
methodsSix hepatocellular carcinoma (HCC) cell lines were examined for different CKs, LN and its receptor by immunocytochemistry and Western blotting. Double immunofluorescent reaction, laser-scanning confocal microscopy and an in vitro induction procedure were used to demonstrate the role of LN in regulating CK19 expression in these cells.
resultsImmunoreactivities for CK8, CK18, CK7 and the receptor for LN were observed in all the six HCC cell lines examined. However, CK19 was merely found in four of the six cell lines, and was in any case associated with LN expression. Laser-scanning confocal microscopy demonstrated the concomitant presence of these two molecules in most of the positive cells. In the two HCC cell lines, originally negative for CK19, addition of LN to the culture medium resulted in an induction of CK19 in a dose-dependent manner. Both the artificially induced and the intrinsic production of CK19 were completely blocked by an antibody to LN.
conclusionLN can induce expression of CK19 in HCC cells in vitro, providing direct evidence for our hypothesis that the abnormal hepatocytic CK19 expression in situ is due to pathologic LN deposition.
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