ArticleOncogene2003
Separation of long-range human TERT gene haplotypes by transformation-associated recombination cloning in yeast.
Article in Oncogene, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Review
- Highly Selective, CRISPR/Cas9-Mediated Isolation of Genes and Genomic Loci from Complex Genomes by TAR Cloning in Yeast.Current protocols · 2021Article
- The hTERT-VNTR2-2Genes & genomics · 2019Article
- TAR Cloning: Perspectives for Functional Genomics, Biomedicine, and Biotechnology.Molecular therapy. Methods & clinical development · 2019Review
- Transformation-associated recombination (TAR) cloning for genomics studies and synthetic biology.Chromosoma · 2016Review
- Highly efficient CRISPR/Cas9-mediated TAR cloning of genes and chromosomal loci from complex genomes in yeast.Nucleic acids research · 2015Article
- Selective isolation of genomic loci from complex genomes by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae.Nature protocols · 2008Article
- An efficient method for multi-locus molecular haplotyping.Nucleic acids research · 2007Article
- Direct molecular haplotyping by melting curve analysis of hybridization probes: beta 2-adrenergic receptor haplotypes as an example.Nucleic acids research · 2005Article
- A novel expression system for genomic DNA loci using a human artificial chromosome vector with transformation-associated recombination cloning.Journal of human genetics · 2005Article
- Closing the gaps on human chromosome 19 revealed genes with a high density of repetitive tandemly arrayed elements.Genome research · 2004Article
- A general cloning system to selectively isolate any eukaryotic or prokaryotic genomic region in yeast.BMC genomics · 2003Article
- Optimum conditions for selective isolation of genes from complex genomes by transformation-associated recombination cloning.Nucleic acids research · 2003Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hTERT gene encoding a catalytic subunit of human telomerase contains four blocks of variable number of tandem repeats (VNTRs)--two in intron 2 and two in intron 6. The segregation of hTERT VNTRs was analysed in families, revealing that all of them were transmitted through meiosis following a Mendelian inheritance. The work reports a further characterization of the minisatellites in hTERT. We employed transformation-associated recombination (TAR) cloning to isolate parental hTERT alleles and determined the specific combination of minisatellites at each of the polymorphic sites. A long-range haplotyping of hTERT determined by TAR cloning was verified by classical Mendelian analysis. Since such a strategy can be applied for any chromosomal locus, we conclude that recombinational gene capture could greatly facilitate haplotypes analysis.
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