ArticleNucleic acids research2003
Analysis and accurate quantification of CpG methylation by MALDI mass spectrometry.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- The Clinical Significance of Promoter Methylation of Fluoropyrimidine Metabolizing and Cyclooxygenase Genes in Colorectal Cancer.Epigenetics insights · 2021Article
- Double signal enhancement strategy based on rolling circle amplification and photoinduced electron transfer for ultrasensitive fluorometric detection of methylated DNA.Mikrochimica acta · 2018Article
- A novel restriction endonuclease GlaI for rapid and highly sensitive detection of DNA methylation coupled with isothermal exponential amplification reaction.Chemical science · 2018Article
- Article
- Genome-wide assays that identify and quantify modified cytosines in human disease studies.Epigenetics & chromatin · 2015Review
- Quantitative DNA methylation analyses reveal stage dependent DNA methylation and association to clinico-pathological factors in breast tumors.BMC cancer · 2013Article
- Application of mass spectrometry to molecular diagnostics of viral infections.Expert review of molecular diagnostics · 2013Review
- DNA methylation analysis in human cancer.Methods in molecular biology (Clifton, N.J.) · 2013Article
- DNA methylation profiling in the clinic: applications and challenges.Nature reviews. Genetics · 2012Review
- A refined, rapid and reproducible high resolution melt (HRM)-based method suitable for quantification of global LINE-1 repetitive element methylation.BMC research notes · 2011Article
- Fluorescent conjugated polymer-based FRET technique for detection of DNA methylation of cancer cells.Nature protocols · 2010Article
- Principles and challenges of genomewide DNA methylation analysis.Nature reviews. Genetics · 2010Review
- Profiling the selectivity of DNA ligases in an array format with mass spectrometry.Nucleic acids research · 2010Article
- Kinetic study of beta-amyloid residue accessibility using reductive alkylation and mass spectrometry.Biotechnology and bioengineering · 2009Article
- Epigenetic and microRNA-mediated regulation in diabetes.Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association · 2009Article
- Technologies in the Whole-Genome Age: MALDI-TOF-Based Genotyping.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2009Article
- Genomic profiling of CpG methylation and allelic specificity using quantitative high-throughput mass spectrometry: critical evaluation and improvements.Nucleic acids research · 2007Article
- A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.Nucleic acids research · 2006Article
- Matrix-assisted laser desorption/ionisation, time-of-flight mass spectrometry in genomics research.PLoS genetics · 2006Review
- Novel method for high throughput DNA methylation marker evaluation using PNA-probe library hybridization and MALDI-TOF detection.Nucleic acids research · 2006Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As the DNA sequence of the human genome is now nearly finished, the main task of genome research is to elucidate gene function and regulation. DNA methylation is of particular importance for gene regulation and is strongly implicated in the development of cancer. Even minor changes in the degree of methylation can have severe consequences. An accurate quantification of the methylation status at any given position of the genome is a powerful diagnostic indicator. Here we present the first assay for the analysis and precise quantification of methylation on CpG positions in simplex and multiplex reactions based on matrix-assisted laser desorption/ ionisation mass spectrometry detection. Calibration curves for CpGs in two genes were established and an algorithm was developed to account for systematic fluctuations. Regression analysis gave R(2) >or= 0.99 and standard deviation around 2% for the different positions. The limit of detection was approximately 5% for the minor isomer. Calibrations showed no significant differences when carried out as simplex or multiplex analyses. All variable parameters were thoroughly investigated, several paraffin-embedded tissue biopsies were analysed and results were verified by established methods like analysis of cloned material. Mass spectrometric results were also compared to chip hybridisation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.