ArticleNucleic acids research2003
RNase T1 mediated base-specific cleavage and MALDI-TOF MS for high-throughput comparative sequence analysis.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 89 citations in OpenAlex.
- Identification and quantification of intracellular N-acetylglucosamine modified glycoRNAs.Nature communications · 2026Article
- Directed Evolution of T7 RNA Polymerase Minimizes dsRNA By-product and Enables High-Fidelity mRNA Synthesis for Demanding Therapeutic Applications.Research (Washington, D.C.) · 2026Article
- Ultrafast enzymatic digestion of deoxyribonucleic acid in aqueous microdroplets for sequence discrimination and identification.QRB discovery · 2021Article
- Article
- Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma.Oncology letters · 2018Article
- Recognition of guanosine by dissimilar tRNA methyltransferases.RNA (New York, N.Y.) · 2012Article
- Dysregulation of DGCR6 and DGCR6L: psychopathological outcomes in chromosome 22q11.2 deletion syndrome.Translational psychiatry · 2012Article
- Two combinatorial optimization problems for SNP discovery using base-specific cleavage and mass spectrometry.BMC systems biology · 2012Article
- Fluorescent labeling of tRNA dihydrouridine residues: Mechanism and distribution.RNA (New York, N.Y.) · 2011Article
- Agmatidine, a modified cytidine in the anticodon of archaeal tRNA(Ile), base pairs with adenosine but not with guanosine.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Identification of RNA molecules by specific enzyme digestion and mass spectrometry: software for and implementation of RNA mass mapping.Nucleic acids research · 2009Article
- High-throughput homogeneous mass cleave assay technology for the diagnosis of autosomal recessive Parkinson's disease.The Journal of molecular diagnostics : JMD · 2008Article
- Mass spectrometry-based detection of transfer RNAs by their signature endonuclease digestion products.RNA (New York, N.Y.) · 2007Article
- A new method for accurate assessment of DNA quality after bisulfite treatment.Nucleic acids research · 2007Article
- Genomic profiling of CpG methylation and allelic specificity using quantitative high-throughput mass spectrometry: critical evaluation and improvements.Nucleic acids research · 2007Article
- Bacterial genotyping by 16S rRNA mass cataloging.BMC bioinformatics · 2006Article
- Microbial identification by mass cataloging.BMC bioinformatics · 2006Article
- Quantitative high-throughput analysis of DNA methylation patterns by base-specific cleavage and mass spectrometry.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Multiplexed discovery of sequence polymorphisms using base-specific cleavage and MALDI-TOF MS.Nucleic acids research · 2005Article
- Rapid analysis of CpG methylation patterns using RNase T1 cleavage and MALDI-TOF.Nucleic acids research · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we devise a new method for high-throughput comparative sequence analysis. The developed protocol comprises a homogeneous in vitro transcription/RNase cleavage system with the accuracy and data acquisition speed of matrix-assisted laser desorption/ionization coupled with time-of-flight mass spectrometry (MALDI-TOF MS). In summary, the target region is PCR amplified using primers tagged with promoter sequences of T7 or SP6 RNA polymerase. Using RNase T1, the in vitro transcripts are base-specifically cleaved at every G-position. This reaction results in a characteristic pattern of fragment masses that is indicative of the original target sequence. To enable high-throughput analysis, samples are processed with automated liquid handling devices and nanoliter amounts are dispensed onto SpectroCHIP arrays for reliable and homogeneous MALDI preparation. This system enables rapid automated comparative sequence analysis for PCR products up to 1 kb in length. We demonstrate the feasibility of the devised method for analysis of single nucleotide polymorphisms (SNPs) and pathogen identification.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.