ArticleInternational journal of legal medicine2003
Real-time PCR quantitation of FE65 a beta-amyloid precursor protein-binding protein after traumatic brain injury in rats.
Article in International journal of legal medicine, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 2 of them syntheses that pooled it.
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Who cites it
11 citing papers in PubMed, 2 syntheses or guidelines pooled it, 33 citations in OpenAlex.
- State-of-the-Art on Wound Vitality Evaluation: A Systematic Review.International journal of molecular sciences · 2022Pooled it
- A Systematic Review of Closed Head Injury Models of Mild Traumatic Brain Injury in Mice and Rats.Journal of neurotrauma · 2019Pooled it
- Analysis of sensitivity and specificity: precise recognition of neutrophils during regeneration of contused skeletal muscle in rats.Forensic sciences research · 2022Article
- The course and prognostic factors of cognitive status after central nervous system trauma: a systematic review protocol.BMJ open · 2017Article
- Time-dependent expression of SNAT2 mRNA in the contused skeletal muscle of rats: a possible marker for wound age estimation.Forensic science, medicine, and pathology · 2013Article
- Molecular pathology of brain edema after severe burns in forensic autopsy cases with special regard to the importance of reference gene selection.International journal of legal medicine · 2013Article
- Stability of endogenous reference genes in postmortem human brains for normalization of quantitative real-time PCR data: comprehensive evaluation using geNorm, NormFinder, and BestKeeper.International journal of legal medicine · 2012Article
- Time-dependent expression of skeletal muscle troponin I mRNA in the contused skeletal muscle of rats: a possible marker for wound age estimation.International journal of legal medicine · 2010Article
- Housekeeping while brain's storming Validation of normalizing factors for gene expression studies in a murine model of traumatic brain injury.BMC molecular biology · 2008Article
- Traumatic brain injury: cause or risk of Alzheimer's disease? A review of experimental studies.Journal of neural transmission (Vienna, Austria : 1996) · 2005Review
- Real-time quantitative PCR assay for the detection of Helicobacter pylori: no association with sudden infant death syndrome.International journal of legal medicine · 2005Article
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10 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
In cases of traumatic brain injury (TBI) in which the patient survived for only a short period of time and was without macroscopic changes at autopsy, it is difficult to diagnose TBI. To detect early diagnostic markers of diffuse axonal injury (DAI), real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) in an experimental head trauma model of rat was chosen. The beta-amyloid precursor protein (beta-APP) is a well-known diagnostic marker of DAI which can be detected by immunolabeling as early as 1.5 h after injury. beta-APP has a binding protein, FE65, which is expressed in the brain of Alzheimer's disease patients along with beta-APP, but no involvement with brain injury has been reported. Neuron-specific enolase (NSE) is also a useful marker of DAI. We found that FE65 expression increased dramatically as early as 30 min after injury and decreased after peaking 1 h post-injury, although NSE showed no significant changes. These results suggest that real-time PCR of FE65 mRNA is useful for the diagnosis of DAI in forensic cases.
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