ArticleClinical & experimental metastasis2003
GFP-transfected tumor cells are useful in examining early metastasis in vivo, but immune reaction precludes long-term tumor development studies in immunocompetent mice.
Article in Clinical & experimental metastasis, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 73 citations in OpenAlex.
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- Leveraging preclinical models of metastatic breast cancer.Biochimica et biophysica acta. Reviews on cancer · 2024Review
- Overcoming Xenoantigen Immunity to Enable Cellular Tracking and Gene Regulation with Immune-competent "NoGlow" Mice.Cancer research communications · 2024Article
- Quantitative Detection of Disseminated Melanoma Cells by Trp-1 Transcript Analysis Reveals Stochastic Distribution of Pulmonary Metastases.Journal of clinical medicine · 2021Article
- Characterization of the role of Samsn1 loss in multiple myeloma development.FASEB bioAdvances · 2020Article
- The utility of the "Glowing Head" mouse for breast cancer metastasis research.Clinical & experimental metastasis · 2020Article
- Targeting myeloid-derived suppressor cells in combination with primary mammary tumor resection reduces metastatic growth in the lungs.Breast cancer research : BCR · 2019Article
- Limiting tumor seeding as a therapeutic approach for metastatic disease.Pharmacology & therapeutics · 2019Review
- Differences between epithelial and mesenchymal human tongue cancer cell lines in experimental metastasis.Oncology letters · 2018Article
- Pre-clinical Orthotopic Murine Model of Human Prostate Cancer.Journal of visualized experiments : JoVE · 2016Article
- Tumorigenicity and Validity of Fluorescence Labelled Mesenchymal and Epithelial Human Oral Cancer Cell Lines in Nude Mice.BioMed research international · 2016Article
- Review
- Metastasis of circulating tumor cells: favorable soil or suitable biomechanics, or both?Cell adhesion & migration · 2015Review
- Drug Development for Metastasis Prevention.Critical reviews in oncogenesis · 2015Review
- Differential arrest and adhesion of tumor cells and microbeads in the microvasculature.Biomechanics and modeling in mechanobiology · 2014Article
- Long-term intravital immunofluorescence imaging of tissue matrix components with epifluorescence and two-photon microscopy.Journal of visualized experiments : JoVE · 2014Article
- "Glowing head" mice: a genetic tool enabling reliable preclinical image-based evaluation of cancers in immunocompetent allografts.PloS one · 2014Article
- Intravital immunofluorescence for visualizing the microcirculatory and immune microenvironments in the mouse ear dermis.PloS one · 2013Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To develop effective therapeutic strategies aimed at treating tumor metastasis, critical steps in this process must be better understood. For this purpose we have established a new model to visualize and quantify early metastasis. Murine CT-26 colon adenocarcinoma cells were stably transfected with green fluorescent protein (GFP). Tumor cells were intraportally delivered to the liver of Balb/c mice and subsequently tracked by intravital fluorescence microscopy. Coinjection of fluorescent beads and in vivo propidium iodide staining allowed examination of initial tumor cell arrest, extravasation, viability and proliferation. Results showed that GFP-transfection compared to conventional labeling procedures (Calcein, cytoplasmic microspheres) did not alter early metastatic properties. However, the long-term development of liver metastases expressing GFP was markedly reduced compared to wild type CT-26 tumor cells. An increase in the size and the number of liver metastases in T- and B-cell-deficient SCID mice suggested an immune response to the GFP transfected cells responsible for the reduced metastatic growth in wild-type mice. Based on our findings, this model can be used to examine the early steps of metastasis in vivo. However, in immunocompetent mice, the use of GFP-labeled tumor cells should be limited to tracking cell arrest and extravasation, whereas evaluations of long-term metastatic growth should be performed in immunodeficient mice.
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Registered trials
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