ArticleGene therapy2003
Role of paracellular junction complexes in baculovirus-mediated gene transfer to nondividing rat hepatocytes.
Article in Gene therapy, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Expression profiling of adhesion proteins during prenatal and postnatal liver development in rats.Stem cells and cloning : advances and applications · 2017Article
- Improving promiscuous mammalian cell entry by the baculovirus Autographa californica multiple nuclear polyhedrosis virus.Bioscience reports · 2012Article
- Interferon-β sensitivity of tumor cells correlates with poor response to VA7 virotherapy in mouse glioma models.Molecular therapy : the journal of the American Society of Gene Therapy · 2012Article
- Baculovirus vectors for gene therapy.Advances in virus research · 2006Review
- Baculovirus as versatile vectors for protein expression in insect and mammalian cells.Nature biotechnology · 2005Review
- Baculovirus as a highly efficient expression vector in insect and mammalian cells.Acta pharmacologica Sinica · 2005Review
- The baculovirus GP64 protein mediates highly stable infectivity of a human respiratory syncytial virus lacking its homologous transmembrane glycoproteins.Journal of virology · 2004Article
- Tumor necrosis factor-alpha acts as a complete mitogen for primary rat hepatocytes.The American journal of pathology · 2003Article
- Expression of E-cadherin and other paracellular junction genes is decreased in iron-loaded hepatocytes.The American journal of pathology · 2003Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Gene delivery to differentiated hepatocytes is notoriously difficult. Hepatocytes plated on collagen-coated dishes and maintained in dimethyl sulfoxide (DMSO)-supplemented medium acquire paracellular junctions, arrange themselves in multicellular islands and are an excellent in vitro model for studying liver function. Baculovirus-mediated gene delivery to hepatocytes in this culture system is restricted to peripheral cells of the islands. However, this limitation can be overcome by transient calcium depletion of the cells prior to and during baculovirus infection. Examination of the mechanism underlying this process revealed that calcium depletion was accompanied by a transient loss of intercellular contacts and paracellular junction complex integrity, increased distance between adjoining cells, and internalization of the tight junction protein, zona occludens ZO-1. Internalization of ZO-1 was accompanied by baculovirus infection of internal cells of hepatocyte islands. When calcium levels were restored, paracellular junction complex integrity returned to normal by 12 h. No permanent alterations in hepatocyte ultrastructure and albumin mRNA, and protein expression were caused by this gene transfer method. Loss in paracellular junction complex integrity exposes the basolateral (sinusoidal) surface of hepatocytes resulting in homogeneous baculovirus-mediated gene delivery to approximately 75% of the cells in long-term DMSO culture. We conclude that the use of recombinant baculovirus as a vector in combination with transient calcium depletion is a highly efficient method for delivering exogenous genes to hepatocytes without loss of hepatic differentiation.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.