ArticleProceedings of the National Academy of Sciences of the United States of America2003
Molecular characterization of the 1,3-propanediol (1,3-PD) operon of Clostridium butyricum.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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59 citing papers in PubMed.
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- The potential of native and engineered Clostridia for biomass biorefining.Frontiers in bioengineering and biotechnology · 2024Review
- Heterologous 1,3-Propanediol Production Using Different RecombinantMicroorganisms · 2023Article
- Activation of Glycyl Radical Enzymes─Multiscale Modeling Insights into Catalysis and Radical Control in a Pyruvate Formate-Lyase-Activating Enzyme.Journal of chemical information and modeling · 2022Article
- The BJournal of inorganic biochemistry · 2022Article
- Bacterial microcompartments for isethionate desulfonation in the taurine-degrading human-gut bacterium Bilophila wadsworthia.BMC microbiology · 2021Article
- Isolation and characterization of a newly identified Clostridium butyricum strain SCUT343-4 for 1,3-propanediol production.Bioprocess and biosystems engineering · 2021Article
- Metabolism, morphology and transcriptome analysis of oscillatory behavior of Clostridium butyricum during long-term continuous fermentation for 1,3-propanediol production.Biotechnology for biofuels · 2020Article
- Development of CRISPR Interference (CRISPRi) Platform for Metabolic Engineering ofInternational journal of molecular sciences · 2020Article
- Recent Progress in the Understanding and Engineering of Coenzyme BFrontiers in bioengineering and biotechnology · 2020Review
- Synergy at work: linking the metabolism of two lactic acid bacteria to achieve superior production of 2-butanol.Biotechnology for biofuels · 2020Article
- l-Rhamnose Metabolism in Clostridium beijerinckii Strain DSM 6423.Applied and environmental microbiology · 2019Article
- Production of 3-Hydroxypropanoic Acid From Glycerol by Metabolically Engineered Bacteria.Frontiers in bioengineering and biotechnology · 2019Review
- Impact of acetolactate synthase inactivation on 1,3-propanediol fermentation by Klebsiella pneumoniae.PloS one · 2019Article
- Metabolic engineering ofBiotechnology for biofuels · 2019Article
- Reviving the Weizmann process for commercial n-butanol production.Nature communications · 2018Article
- Role ofFrontiers in microbiology · 2018Review
- New tricks for the glycyl radical enzyme family.Critical reviews in biochemistry and molecular biology · 2017Review
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5 authors.
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Abstract
The genes encoding the 1,3-propanediol (1,3-PD) operon of Clostridium butyricum VPI1718 were characterized from a molecular and a biochemical point of view. This operon is composed of three genes, dhaB1, dhaB2, and dhaT. When grown in a vitamin B12-free mineral medium with glycerol as carbon source, Escherichia coli expressing dhaB1, dhaB2, and dhaT produces 1,3-PD and high glycerol dehydratase and 1,3-PD dehydrogenase activities. dhaB1 and dhaB2 encode, respectively, a new type of glycerol dehydratase and its activator protein. The deduced proteins DhaB1 and DhaB2, with calculated molecular masses of 88,074 and 34,149 Da, respectively, showed no homology with the known glycerol dehydratases that are all B12 dependent but significant similarity with the pyruvate formate lyases and pyruvate formate lyases activating enzymes and their homologues. The 1,158-bp dhaT gene codes for a 1,3-PD dehydrogenase with a calculated molecular mass of 41,558 Da, revealing a high level of identity with other DhaT proteins from natural 1,3-PD producers. The expression of the 1,3-PD operon in C. butyricum is regulated at the transcriptional level, and this regulation seems to involve a two-component signal transduction system DhaASDhaA, which may have a similar function to DhaR, a transcriptional regulator found in other natural 1,3-PD producers. The discovery of a glycerol dehydratase, coenzyme B12 independent, should significantly influence the development of an economical vitamin B12-free biological process for the production of 1,3-PD from renewable resources.
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