ArticleRNA (New York, N.Y.)2003
Analysis of the stimulatory effect of splicing on mRNA production and utilization in mammalian cells.
Article in RNA (New York, N.Y.), 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
102 citing papers in PubMed, 164 citations in OpenAlex.
- Coordinating mRNA maturation: The U1 relay model.Molecular cell · 2026Review
- Comprehensive analysis of the role of ASC1 and APE2 introns on cellular fitness, transcription, and post-transcriptional dynamics.The FEBS journal · 2026Article
- Barriers to the Pharmacologic Rescue of W1282X CFTR.Biochemistry · 2026Article
- Article
- A Y-linked duplication of anti-Mullerian hormone is the sex determination gene in threespine stickleback.PLoS genetics · 2025Article
- Minigenes enhance heterologous expression and prevent aberrant splicing of mouse Spink1.Scientific reports · 2025Article
- Alternative splicing-mediated regulation of a novel allele of Badh2 drives moderate accumulation of 2-AP in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Introns increase gene expression in Caenorhabditis elegans by a mechanism that must be at least partly different than in plants.Scientific reports · 2025Article
- Sequence-dependent and -independent effects of intron-mediated enhancement learned from thousands of random introns.Nucleic acids research · 2025Article
- Introns and Their Therapeutic Applications in Biomedical Researches.Iranian journal of biotechnology · 2023Review
- Calumenin knockdown, by intronic artificial microRNA, to improve expression efficiency of the recombinant human coagulation factor IX.Biotechnology letters · 2022Article
- Fine-mapping and identification of a candidate gene controlling seed coat color in melon (Cucumis melo L. var. chinensis Pangalo).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Cues from mRNA splicing prevent default Argonaute silencing in C. elegans.Developmental cell · 2021Article
- Co-transcriptional splicing regulates 3' end cleavage during mammalian erythropoiesis.Molecular cell · 2021Article
- Proximity to the Promoter and Terminator Regions Regulates the Transcription Enhancement Potential of an Intron.Frontiers in molecular biosciences · 2021Article
- Novel InDels ofAnimals : an open access journal from MDPI · 2020Article
- Fluorescent protein expression in the ectomycorrhizal fungus Laccaria bicolor: a plasmid toolkit for easy use of fluorescent markers in basidiomycetes.Current genetics · 2020Article
- Expression vector cassette engineering for recombinant therapeutic production in mammalian cell systems.Applied microbiology and biotechnology · 2020Review
- The mRNA encoding the JUND tumor suppressor detains nuclear RNA-binding proteins to assemble polysomes that are unaffected by mTOR.The Journal of biological chemistry · 2020Article
- A Rev-CBP80-eIF4AI complex drives Gag synthesis from the HIV-1 unspliced mRNA.Nucleic acids research · 2018Article
42 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have examined how splicing affects the expression of a range of human and nonhuman genes in vertebrate cells. Although our data demonstrate that splicing invariably enhances the level of gene expression, this positive effect is generally moderate. However, in the case of the human beta-globin gene, splicing is essential for significant protein expression. In the absence of introns, 3' end processing is inefficient, and this appears to be causally linked to a significant decrease in the level of both nuclear and cytoplasmic 3' end-processed RNA. In contrast, splicing appears to only modestly enhance nuclear mRNA export. Consistent with this observation, intronless beta-globin gene expression was only partially rescued by the insertion of retroviral nuclear mRNA export elements. Surprisingly, in the case of the highly intron dependent beta-globin gene, the mRNA that did reach the cytoplasm was also only inefficiently translated if it derived from an intronless expression plasmid. Together, these data argue that splicing can increase gene expression by enhancing mRNA 3' end processing, and hence, mRNA production. Moreover, in the case of the highly intron-dependent beta-globin gene, splicing also significantly enhanced the translational utilization of cytoplasmic beta-globin mRNAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.