ArticleProceedings of the National Academy of Sciences of the United States of America2003
Gamma 2 subunit of G protein heterotrimer is an N-end rule ubiquitylation substrate.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- The Deubiquitylase Otub1 Regulates the Chemotactic Response of Splenic B Cells by Modulating the Stability of the γ-Subunit Gng2.Molecular and cellular biology · 2024Article
- Minireview: ubiquitination-regulated G protein-coupled receptor signaling and trafficking.Molecular endocrinology (Baltimore, Md.) · 2013Review
- HIV-1 Nef impairs heterotrimeric G-protein signaling by targeting Gα(i2) for degradation through ubiquitination.The Journal of biological chemistry · 2012Article
- Arginylated calreticulin at plasma membrane increases susceptibility of cells to apoptosis.The Journal of biological chemistry · 2012Article
- The N-end rule pathway: emerging functions and molecular principles of substrate recognition.Nature reviews. Molecular cell biology · 2011Review
- Pheromone- and RSP5-dependent ubiquitination of the G protein beta subunit Ste4 in yeast.The Journal of biological chemistry · 2011Article
- The N-end rule pathway and regulation by proteolysis.Protein science : a publication of the Protein Society · 2011Review
- Binding of β4γ5 by adenosine A1 and A2A receptors determined by stable isotope labeling with amino acids in cell culture and mass spectrometry.Biochemistry · 2011Article
- G Protein Mono-ubiquitination by the Rsp5 Ubiquitin Ligase.The Journal of biological chemistry · 2009Article
- Structural determinants involved in the formation and activation of G protein betagamma dimers.Neuro-Signals · 2009Review
- Killing of macrophages by anthrax lethal toxin: involvement of the N-end rule pathway.Cellular microbiology · 2008Article
- Seven-transmembrane receptors and ubiquitination.Circulation research · 2007Review
- Post-translational arginylation of calreticulin: a new isospecies of calreticulin component of stress granules.The Journal of biological chemistry · 2007Article
- Loss of Ubr1 promotes aneuploidy and accelerates B-cell lymphomagenesis in TLX1/HOX11-transgenic mice.Oncogene · 2006Article
- Protein arginylation in rat brain cytosol: a proteomic analysis.Neurochemical research · 2006Article
- A family of mammalian E3 ubiquitin ligases that contain the UBR box motif and recognize N-degrons.Molecular and cellular biology · 2005Article
- Female lethality and apoptosis of spermatocytes in mice lacking the UBR2 ubiquitin ligase of the N-end rule pathway.Molecular and cellular biology · 2003Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterotrimeric G proteins transduce signals from activated transmembrane G protein-coupled receptors to appropriate downstream effectors within cells. Signaling specificity is achieved in part by the specific alpha, beta, and gamma subunits that compose a given heterotrimer. Additional structural and functional diversity in these subunits is generated at the level of posttranslational modification, offering alternate regulatory mechanisms for G protein signaling. Presented here is the identification of a variant of the gamma(2) subunit of G protein heterotrimer purified from bovine brain and the demonstration that this RDTASIA gamma(2) variant, containing unique amino acid sequence at its N terminus, is a substrate for ubiquitylation and degradation via the N-end rule pathway. Although N-end-dependent degradation has been shown to have important functions in peptide import, chromosome segregation, angiogenesis, and cardiovascular development, the identification of cellular substrates in mammalian systems has remained elusive. The isolation of RDTASIA gamma(2) from a native tissue represents identification of a mammalian N-end rule substrate from a physiological source, and elucidates a mechanism for the targeting of G protein gamma subunits for ubiquitylation and degradation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.