ArticleJournal of bacteriology2003
Atypical 16S rRNA gene copies in Ochrobactrum intermedium strains reveal a large genomic rearrangement by recombination between rrn copies.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 55 citations in OpenAlex.
- Delineation of Taxonomic Species within Complex of Species:Frontiers in microbiology · 2017Article
- Highly DiversifiedFrontiers in microbiology · 2017Article
- High Prevalence of SXT/R391-Related Integrative and Conjugative Elements Carrying blaCMY-2 in Proteus mirabilis Isolates from Gulls in the South of France.Antimicrobial agents and chemotherapy · 2016Article
- Niches, population structure and genome reduction in Ochrobactrum intermedium: clues to technology-driven emergence of pathogens.PloS one · 2014Article
- Genetic characterization of atypical Citrobacter freundii.PloS one · 2013Article
- From environment to man: genome evolution and adaptation of human opportunistic bacterial pathogens.Genes · 2012Article
- Gluconobacter as well as Asaia species, newly emerging opportunistic human pathogens among acetic acid bacteria.Journal of clinical microbiology · 2010Article
- Dual detection of Legionella pneumophila and Legionella species by real-time PCR targeting the 23S-5S rRNA gene spacer region.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2010Article
- Plastic architecture of bacterial genome revealed by comparative genomics of Photorhabdus variants.Genome biology · 2008Article
- Intragenomic variation and evolution of the internal transcribed spacer of the rRNA operon in bacteria.Journal of molecular evolution · 2007Article
- A semi-quantitative GeLC-MS analysis of temporal proteome expression in the emerging nosocomial pathogen Ochrobactrum anthropi.Genome biology · 2007Article
- Evolutionary and diagnostic implications of intragenomic heterogeneity in the 16S rRNA gene in Aeromonas strains.Journal of bacteriology · 2005Article
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5 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Ochrobactrum intermedium is an opportunistic human pathogen belonging to the alpha 2 subgroup of proteobacteria. The 16S rDNA sequences of nine O. intermedium isolates from a collection of clinical and environmental isolates exhibited a 46-bp insertion at position 187, which was present in only one sequence among the 82 complete or partial 16S rDNA sequences of Ochrobactrum spp. available in data banks. Reverse transcription-PCR experiments showed that the 46-bp insertion remained in the 16S rRNA. The inserted sequence folded into a stem-loop structure, which took place in and prolonged helix H184 of the 16S rRNA molecule. Helix H184 has been described as conserved in length among eubacteria, suggesting the idiosyncratic character of the 46-bp insertion. Pulsed-field gel electrophoresis experiments showed that seven of the clinical isolates carrying the 46-bp insertion belonged to the same clone. Insertion and rrn copy numbers were determined by hybridization and I-CeuI digestion. In the set of clonal isolates, the loss of two insertion copies revealed the deletion of a large genomic fragment of 150 kb, which included one rrn copy; deletion occurred during the in vivo evolution of the clone. Determination of the rrn skeleton suggested that the large genomic rearrangement occurred during events involving homologous recombination between rrn copies. The loss of insertion copies suggested a phenomenon of concerted evolution among heterogeneous rrn copies.
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