ArticleJournal of bacteriology2003
Natural variation in the microcystin synthetase operon mcyABC and impact on microcystin production in Microcystis strains.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
80 citing papers in PubMed, 243 citations in OpenAlex.
- Secondary metabolism ofApplied and environmental microbiology · 2026Review
- Article
- Unprecedented toxic blooms of Microcystis spp. in 2019 in the Chowan River, North Carolina.Harmful algae · 2024Article
- Synthesis of a Truncated Microcystin Tetrapeptide Molecule from a Partial Mcy Gene Cluster in Microcystis Cultures and Blooms.Environmental science & technology · 2024Article
- Effects of salinity and nutrient stress on a toxic freshwater cyanobacterial community and its associated microbiome: An experimental study.Environmental microbiology reports · 2024Article
- Article
- Article
- Duplex Real-Time Fluorescent Quantitative PCR Assays for the Detection of Toxigenic Microcystis Genotypes Based on SNP/InDel Variation in mcy Gene Cluster.Current microbiology · 2024Article
- Biological and Chemical Approaches for Controlling Harmful Microcystis Blooms.Journal of microbiology (Seoul, Korea) · 2024Review
- Microcystin congeners in Lake Erie follow the seasonal pattern of nitrogen availability.Harmful algae · 2023Article
- Cyanotoxins, biosynthetic gene clusters, and factors modulating cyanotoxin biosynthesis.World journal of microbiology & biotechnology · 2023Review
- Microcystin Contamination and Toxicity: Implications for Agriculture and Public Health.Toxins · 2022Review
- Metagenomic and Metatranscriptomic Insights into Population Diversity ofApplied and environmental microbiology · 2022Article
- Morphological, molecular, and biochemical study of cyanobacteria from a eutrophic Algerian reservoir (Cheffia).Environmental science and pollution research international · 2022Article
- Genotyping and Multivariate Regression Trees Reveal Ecological Diversification within the Microcystis aeruginosa Complex along a Wide Environmental Gradient.Applied and environmental microbiology · 2022Article
- Ganga river water quality assessment using combined approaches: physico-chemical parameters and cyanobacterial toxicity detection with special reference to microcystins and molecular characterization of microcystin synthetase (mcy) genes carrying cyanobacteria.Environmental science and pollution research international · 2022Article
- Article
- Characterization and Diversity of Microcystins Produced by Cyanobacteria from the Curonian Lagoon (SE Baltic Sea).Toxins · 2021Article
- Differential Labeling of Chemically Modified Peptides and Lipids among CyanobacteriaMicroorganisms · 2021Article
- Absence of Cyanotoxins in Llayta, Edible Nostocaceae Colonies from the Andes Highlands.Toxins · 2020Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Toxic Microcystis strains often produce several isoforms of the cyclic hepatotoxin microcystin, and more than 65 isoforms are known. This has been attributed to relaxed substrate specificity of the adenylation domain. Our results show that in addition to this, variability is also caused by genetic variation in the microcystin synthetase genes. Genetic characterization of a region of the adenylation domain in module mcyB1 resulted in identification of two groups of genetic variants in closely related Microcystis strains. Sequence analyses suggested that the genetic variation is due to recombination events between mcyB1 and the corresponding domains in mcyC. Each variant could be correlated to a particular microcystin isoform profile, as identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Among the Microcystis species studied, we found 11 strains containing different variants of the mcyABC gene cluster and 7 strains lacking the genes. Furthermore, there is no concordance between the phylogenies generated with mcyB1, 16S ribosomal DNA, and DNA fingerprinting. Collectively, these results suggest that recombination between imperfect repeats, gene loss, and horizontal gene transfer can explain the distribution and variation within the mcyABC operon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.