ArticleMolecular and cellular biology2003
A 1-megadalton ESC/E(Z) complex from Drosophila that contains polycomblike and RPD3.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 130 citations in OpenAlex.
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- Polycomb-like 2 regulates PRC2 components to affect proliferation in glioma cells.Journal of neuro-oncology · 2020Article
- A Dimeric Structural Scaffold for PRC2-PCL Targeting to CpG Island Chromatin.Molecular cell · 2020Article
- Differential Contributions of DNA-Binding Proteins to Polycomb Response Element Activity at theGenetics · 2020Article
- Transcriptome Analysis of Long-lived Drosophila melanogaster E(z) Mutants Sheds Light on the Molecular Mechanisms of Longevity.Scientific reports · 2019Article
- PHD finger protein 1 (PHF1) is a novel reader for histone H4R3 symmetric dimethylation and coordinates with PRMT5-WDR77/CRL4B complex to promote tumorigenesis.Nucleic acids research · 2018Article
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- PHF1 is required for chromosome alignment and asymmetric division during mouse meiotic oocyte maturation.Cell cycle (Georgetown, Tex.) · 2018Article
- Polycomb and Trithorax Group Genes inGenetics · 2017Review
- Polycomb inhibits histone acetylation by CBP by binding directly to its catalytic domain.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The WD40 Domain Protein MSI1 Functions in a Histone Deacetylase Complex to Fine-Tune Abscisic Acid Signaling.The Plant cell · 2016Article
- The recruitment of chromatin modifiers by long noncoding RNAs: lessons from PRC2.RNA (New York, N.Y.) · 2015Review
- Distinct Cellular Assembly Stoichiometry of Polycomb Complexes on Chromatin Revealed by Single-molecule Chromatin Immunoprecipitation Imaging.The Journal of biological chemistry · 2015Article
- Sex comb on midleg (Scm) is a functional link between PcG-repressive complexes in Drosophila.Genes & development · 2015Article
- A dimeric state for PRC2.Nucleic acids research · 2014Article
- Global regulation of mRNA translation and stability in the early Drosophila embryo by the Smaug RNA-binding protein.Genome biology · 2014Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Polycomb group (PcG) proteins are required to maintain stable repression of the homeotic genes and others throughout development. The PcG proteins ESC and E(Z) are present in a prominent 600-kDa complex as well as in a number of higher-molecular-mass complexes. Here we identify and characterize a 1-MDa ESC/E(Z) complex that is distinguished from the 600-kDa complex by the presence of the PcG protein Polycomblike (PCL) and the histone deacetylase RPD3. In addition, the 1-MDa complex shares with the 600-kDa complex the histone binding protein p55 and the PcG protein SU(Z)12. Coimmunoprecipitation assays performed on embryo extracts and gel filtration column fractions indicate that, during embryogenesis E(Z), SU(Z)12, and p55 are present in all ESC complexes, while PCL and RPD3 are associated with ESC, E(Z), SU(Z)12, and p55 only in the 1-MDa complex. Glutathione transferase pulldown assays demonstrate that RPD3 binds directly to PCL via the conserved PHD fingers of PCL and the N terminus of RPD3. PCL and E(Z) colocalize virtually completely on polytene chromosomes and are associated with a subset of RPD3 sites. As previously shown for E(Z) and RPD3, PCL and SU(Z)12 are also recruited to the insertion site of a minimal Ubx Polycomb response element transgene in vivo. Consistent with these biochemical and cytological results, Rpd3 mutations enhance the phenotypes of Pcl mutants, further indicating that RPD3 is required for PcG silencing and possibly for PCL function. These results suggest that there may be multiple ESC/E(Z) complexes with distinct functions in vivo.
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