ArticleThe Biochemical journal2003
Induction of glycosylation in human C-reactive protein under different pathological conditions.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it.
- C-reactive protein as a prognostic factor for human osteosarcoma: a meta-analysis and literature review.PloS one · 2014Pooled it
- C-Reactive Protein: Pathophysiology, Diagnosis, False Test Results and a Novel Diagnostic Algorithm for Clinicians.Diseases (Basel, Switzerland) · 2023Review
- Gene expression of the immunoinflammatory and immunological status of obese dogs before and after weight loss.PloS one · 2020Article
- Clinical Proteomics Profiling for Biomarker Identification Among Patients Suffering With Indian Post Kala Azar Dermal Leishmaniasis.Frontiers in cellular and infection microbiology · 2020Article
- The Multiple Faces of C-Reactive Protein-Physiological and Pathophysiological Implications in Cardiovascular Disease.Molecules (Basel, Switzerland) · 2019Review
- Evolution of C-Reactive Protein.Frontiers in immunology · 2019Review
- Mahanine drives pancreatic adenocarcinoma cells into endoplasmic reticular stress-mediated apoptosis through modulating sialylation process and CaScientific reports · 2018Article
- Article
- An epidemiological analysis of potential associations between C-reactive protein, inflammation, and prostate cancer in the male US population using the 2009-2010 National Health and Nutrition Examination Survey (NHANES) data.Frontiers in chemistry · 2015Article
- Sialylation of outer membrane porin protein D: a mechanistic basis of antibiotic uptake in Pseudomonas aeruginosa.Molecular & cellular proteomics : MCP · 2014Article
- Obesity related alterations in plasma cytokines and metabolic hormones in chimpanzees.International journal of inflammation · 2014Article
- C-reactive protein and the biology of disease.Immunologic research · 2013Review
- Article
- α(1)-Acid glycoprotein up-regulates CD163 via TLR4/CD14 protein pathway: possible protection against hemolysis-induced oxidative stress.The Journal of biological chemistry · 2012Article
- Discovery of serum biomarkers of alcoholic fatty liver in a rodent model: C-reactive protein.Journal of biomedical science · 2011Article
- Glycosylation of erythrocyte spectrin and its modification in visceral leishmaniasis.PloS one · 2011Article
- Disease-associated glycosylated molecular variants of human C-reactive protein activate complement-mediated hemolysis of erythrocytes in tuberculosis and Indian visceral leishmaniasis.Glycoconjugate journal · 2009Article
- CRP identifies homeostatic immune oscillations in cancer patients: a potential treatment targeting tool?Journal of translational medicine · 2009Review
- Top-down identification and quantification of stable isotope labeled proteins from Aspergillus flavus using online nano-flow reversed-phase liquid chromatography coupled to a LTQ-FTICR mass spectrometer.Analytical chemistry · 2008Article
- Role of C-reactive protein in complement-mediated hemolysis in Malaria.Glycoconjugate journal · 2006Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As an acute-phase protein, human C-reactive protein (CRP) is clinically important. CRPs were purified from several samples in six different pathological conditions, where their levels ranged from 22 to 342 microg/ml. Small, but significant, variations in electrophoretic mobilities on native PAGE suggested differences in molecular mass, charge and/or shape. Following separation by SDS/PAGE, they showed single subunits with some differences in their molecular masses ranging between 27 and 30.5 kDa, but for a particular disease, the mobility was the same for CRPs purified from multiple individuals or pooled sera. Isoelectric focusing (IEF) also indicated that the purified CRPs differed from each other. Glycosylation was demonstrated in these purified CRPs by Digoxigenin kits, neuraminidase treatment and binding with lectins. The presence of N-linked sugar moiety was confirmed by N-glycosidase F digestion. The presence of sialic acid, glucose, galactose and mannose has been demonstrated by gas liquid chromatography, mass spectroscopic and fluorimetric analysis. Matrix-assisted laser-desorption ionization analysis of the tryptic digests of three CRPs showed systematic absence of two peptide fragments, one at the N-terminus and the other near the C-terminus. Model-building suggested that the loss of these fragments exposed two potential glycosylation sites on a cleft floor keeping the protein-protein interactions in pentraxins and calcium-dependent phosphorylcholine-binding qualitatively unaffected. Thus we have convincingly demonstrated that human CRP is glycosylated in some pathological conditions.
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