ArticleThe Biochemical journal2003
Characterization of XYN10B, a modular xylanase from the ruminal protozoan Polyplastron multivesiculatum, with a family 22 carbohydrate-binding module that binds to cellulose.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 60 citations in OpenAlex.
- A diverse set of Enterococcus-infecting phage provides insight into phage host-range determinants.Virus research · 2024Article
- The release and catabolism of ferulic acid in plant cell wall by rumen microbes: A review.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Review
- Rumen Protozoa Play a Significant Role in Fungal Predation and Plant Carbohydrate Breakdown.Frontiers in microbiology · 2020Article
- Islands in the stream: from individual to communal fiber degradation in the rumen ecosystem.FEMS microbiology reviews · 2019Review
- The extracellular endo-β-1,4-xylanase with multidomain from the extreme thermophileBiotechnology for biofuels · 2019Article
- Molecular cloning, purification, expression, and characterization of β-1, 4-endoglucanase gene (Asian-Australasian journal of animal sciences · 2018Article
- Repeated inoculation of cattle rumen with bison rumen contents alters the rumen microbiome and improves nitrogen digestibility in cattle.Scientific reports · 2017Article
- The Role of Ciliate Protozoa in the Rumen.Frontiers in microbiology · 2015Review
- Activity-based metagenomic screening and biochemical characterization of bovine ruminal protozoan glycoside hydrolases.Applied and environmental microbiology · 2011Article
- Comparative characterization of deletion derivatives of the modular xylanase XynA of Thermotoga maritima.Extremophiles : life under extreme conditions · 2006Article
- Isolation and expression of the xynB gene and its product, XynB, a consistent component of the Clostridium cellulovorans cellulosome.Journal of bacteriology · 2004Article
- Unusual microbial xylanases from insect guts.Applied and environmental microbiology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new xylanase gene, xyn10B, was isolated from the ruminal protozoan Polyplastron multivesiculatum and the gene product was characterized. XYN10B is the first protozoan family 10 glycoside hydrolase characterized so far and is a modular enzyme comprising a family 22 carbohydrate-binding module (CBM) preceding the catalytic domain. The CBM22 was shown to be a true CBM. It showed high affinity for soluble arabinoxylan and is the first example of a CBM22 that binds strongly to celluloses of various crystallinities. The enzymic properties of XYN10B were also analysed. Its optimal temperature and pH for activity were 39 degrees C and 7.0 respectively; these values being close to those of the ruminal ecosystem. The phylogenetic relationships between the XYN10B CBM22 or catalytic domain and related sequences from ruminal and non-ruminal bacteria and eukaryotes are reported. The xyn10B gene is shown to lack introns.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.