ArticleHistochemistry and cell biology2003
Cellular distribution of lens epithelium-derived growth factor (LEDGF) in the rat eye: loss of LEDGF from nuclei of differentiating cells.
Article in Histochemistry and cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- DNA methylation as an epigenetic mechanism in the regulation of LEDGF expression and biological response in aging and oxidative stress.Cell death discovery · 2024Article
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- Inverse dose-rate effect of ionising radiation on residual 53BP1 foci in the eye lens.Scientific reports · 2019Article
- FGF2 antagonizes aberrant TGFβ regulation of tropomyosin: role for posterior capsule opacity.Journal of cellular and molecular medicine · 2017Article
- The role of Prdx6 in the protection of cells of the crystalline lens from oxidative stress induced by UV exposure.Japanese journal of ophthalmology · 2016Article
- The significance of autoantibodies to DFS70/LEDGFp75 in health and disease: integrating basic science with clinical understanding.Clinical and experimental medicine · 2016Review
- The Structure-Specific Recognition Protein 1 Associates with Lens Epithelium-Derived Growth Factor Proteins and Modulates HIV-1 Replication.Journal of molecular biology · 2016Article
- PSIP1/LEDGF: a new gene likely involved in sensorineural progressive hearing loss.Scientific reports · 2015Article
- Dynamic and differential regulation in the microRNA expression in the developing and mature cataractous rat lens.Journal of cellular and molecular medicine · 2013Article
- Elevated tropomyosin expression is associated with epithelial-mesenchymal transition of lens epithelial cells.Journal of cellular and molecular medicine · 2013Article
- Lens epithelium-derived growth factor deSumoylation by Sumo-specific protease-1 regulates its transcriptional activation of small heat shock protein and the cellular response.The FEBS journal · 2012Article
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- Protein expression profiling of lens epithelial cells from Prdx6-depleted mice and their vulnerability to UV radiation exposure.American journal of physiology. Cell physiology · 2010Article
- A hierarchy of proliferative cells exists in mouse lens epithelium: implications for lens maintenance.Investigative ophthalmology & visual science · 2006Article
- Gene expression profiling of diabetic and galactosaemic cataractous rat lens by microarray analysis.Diabetologia · 2005Article
- Innovative techniques and applications in histochemistry and cell biology.Histochemistry and cell biology · 2003Review
- Cell death in health and disease.Journal of cellular and molecular medicineReview
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
Lens epithelium-derived growth factor (LEDGF) enhances the survival and growth of cells. To understand LEDGF's spatial localization and its putative function(s) during proliferation and differentiation, we localized LEDGF during terminal differentiation in whole rat lenses, lens epithelial cell (LEC) explants stimulated with FGF-2, and insulin, iris, human LECs with lentoids. In addition, intracellular localization of LEDGF was performed in other ocular tissues: ciliary body, retina, and cornea. We found the immunopositivity of nuclear LEDGF decreased in LECs of the equatorial region. In contrast, immunopositivity of LEDGF was detected in the cytoplasm of LECs and superficial fiber cells. After treating LEC explants with FGF-2 and insulin, which are known to be differentiating factors for LECs, the nuclei of these cells showed no LEDGF immunopositivity, but explants did express p57(kip2), a differentiation marker protein. Also, immunopositive LEDGF was not detected in the nuclei of differentiated cells, lentoid body, and corneal epithelial cells. This demonstrated that the loss of LEDGF from the nucleus may be associated with the process of terminal differentiation that might be in some way common with the biochemical mechanisms of apoptosis. The spatial and temporal distribution of LEDGF in the present study also provides a vision for further investigation as to how this protein is involved in cell fate determination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.