ArticlePlant physiology2003
Functional analysis of cystathionine gamma-synthase in genetically engineered potato plants.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 90 citations in OpenAlex.
- OverexpressingInternational journal of molecular sciences · 2023Article
- Genetic architecture of tuber-bound free amino acids in potato and effect of growing environment on the amino acid content.Scientific reports · 2023Article
- Cysteine and Methionine Biosynthetic Enzymes Have Distinct Effects on Seed Nutritional Quality and on Molecular Phenotypes Associated With Accumulation of a Methionine-Rich Seed Storage Protein in Rice.Frontiers in plant science · 2020Article
- Alteration of S-adenosylhomocysteine levels affects lignin biosynthesis in switchgrass.Plant biotechnology journal · 2018Article
- Overexpression of serine acetyltransferase in maize leaves increases seed-specific methionine-rich zeins.Plant biotechnology journal · 2018Article
- Engineering sulfur storage in maize seed proteins without apparent yield loss.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The Interplay between Sulfur and Iron Nutrition in Tomato.Plant physiology · 2015Article
- Arabidopsis PIAL1 and 2 promote SUMO chain formation as E4-type SUMO ligases and are involved in stress responses and sulfur metabolism.The Plant cell · 2014Article
- Transcriptome and metabolome analysis of plant sulfate starvation and resupply provides novel information on transcriptional regulation of metabolism associated with sulfur, nitrogen and phosphorus nutritional responses in Arabidopsis.Frontiers in plant science · 2014Article
- Tobacco seeds expressing feedback-insensitive cystathionine gamma-synthase exhibit elevated content of methionine and altered primary metabolic profile.BMC plant biology · 2013Article
- Salt-responsive ERF1 regulates reactive oxygen species-dependent signaling during the initial response to salt stress in rice.The Plant cell · 2013Article
- Transcripts of sulphur metabolic genes are co-ordinately regulated in developing seeds of common bean lacking phaseolin and major lectins.Journal of experimental botany · 2012Article
- Perturbation of Arabidopsis amino acid metabolism causes incompatibility with the adapted biotrophic pathogen Hyaloperonospora arabidopsidis.The Plant cell · 2011Article
- Identification of genes in the phenylalanine metabolic pathway by ectopic expression of a MYB transcription factor in tomato fruit.The Plant cell · 2011Article
- Characterization of the branched-chain amino acid aminotransferase enzyme family in tomato.Plant physiology · 2010Article
- Supply of sulphur to S-deficient young barley seedlings restores their capability to cope with iron shortage.Journal of experimental botany · 2010Article
- Arabidopsis putative selenium-binding protein1 expression is tightly linked to cellular sulfur demand and can reduce sensitivity to stresses requiring glutathione for tolerance.Plant physiology · 2009Article
- Arabidopsis methionine gamma-lyase is regulated according to isoleucine biosynthesis needs but plays a subordinate role to threonine deaminase.Plant physiology · 2009Article
- The Arabidopsis onset of leaf death5 mutation of quinolinate synthase affects nicotinamide adenine dinucleotide biosynthesis and causes early ageing.The Plant cell · 2008Article
- Improving the content of essential amino acids in crop plants: goals and opportunities.Plant physiology · 2008Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants, metabolic pathways leading to methionine (Met) and threonine diverge at the level of their common substrate, O-phosphohomoserine (OPHS). To investigate the regulation of this branch point, we engineered transgenic potato (Solanum tuberosum) plants affected in cystathionine gamma-synthase (CgS), the enzyme utilizing OPHS for the Met pathway. Plants overexpressing potato CgS exhibited either: (a) high transgene RNA levels and 2.7-fold elevated CgS activities but unchanged soluble Met levels, or (b) decreased transcript amounts and enzyme activities (down to 7% of wild-type levels). In leaf tissues, these cosuppression lines revealed a significant reduction of soluble Met and an accumulation of OPHS. Plants expressing CgS antisense constructs exhibited reductions in enzyme activity to as low as 19% of wild type. The metabolite contents of these lines were similar to those of the CgS cosuppression lines. Surprisingly, neither increased nor decreased CgS activity led to visible phenotypic alterations or significant changes in protein-bound Met levels in transgenic potato plants, indicating that metabolic flux to Met synthesis was not greatly affected. Furthermore, in vitro feeding experiments revealed that potato CgS is not subject to feedback regulation by Met, as reported for Arabidopsis. In conclusion, our results demonstrate that potato CgS catalyzes a near-equilibrium reaction and, more importantly, does not display features of a pathway-regulating enzyme. These results are inconsistent with the current hypothesis that CgS exerts major Met metabolic flux control in higher plants.
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