ArticlePlant physiology2003
New insight into Phaeodactylum tricornutum fatty acid metabolism. Cloning and functional characterization of plastidial and microsomal delta12-fatty acid desaturases.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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57 citing papers in PubMed, 149 citations in OpenAlex.
- The life cycle of fatty acids in oleaginous stramenopiles: from synthesis to maturation and beyond.BMC plant biology · 2026Review
- Decoupling of plastid and endomembrane homeoviscous response to low temperature and darkness in Phaeodactylum tricornutum.BMC plant biology · 2025Article
- Diatom triacylglycerol metabolism: from carbon fixation to lipid droplet degradation.Biological reviews of the Cambridge Philosophical Society · 2025Review
- ASAFind 2.0: multi-class protein targeting prediction for diatoms and algae with complex plastids.The Plant journal : for cell and molecular biology · 2025Article
- Characterization of Delta-7 Alkenone Desaturase in Haptophyte Gephyrocapsa huxleyi Through Heterologous Expression in Tisochrysis lutea.Marine biotechnology (New York, N.Y.) · 2025Article
- Acyl-CoA: lysophosphatidylcholine acyltransferase from diatom P. Tricornutum efficiently remodels phosphatidylcholine containing polyunsaturated fatty acids.Scientific reports · 2024Article
- Molecular cloning and functional analysis of a plastidial ω3 desaturase fromFrontiers in microbiology · 2024Article
- Membrane fatty acid desaturase: biosynthesis, mechanism, and architecture.Applied microbiology and biotechnology · 2022Review
- Key Enzymes in Fatty Acid Synthesis Pathway for Bioactive Lipids Biosynthesis.Frontiers in nutrition · 2022Review
- Characterization of fatty acid desaturases reveals stress-induced synthesis of C18 unsaturated fatty acids enriched in triacylglycerol in the oleaginous alga Chromochloris zofingiensis.Biotechnology for biofuels · 2021Article
- Compound-specific stable hydrogen isotope (δRapid communications in mass spectrometry : RCM · 2021Article
- Diatoms and Plants Acyl-CoA:lysophosphatidylcholine Acyltransferases (LPCATs) Exhibit Diverse Substrate Specificity and Biochemical Properties.International journal of molecular sciences · 2021Article
- A non-canonical Δ9-desaturase synthesizing palmitoleic acid identified in the thraustochytrid Aurantiochytrium sp. T66.Applied microbiology and biotechnology · 2021Article
- Microalgae as Sustainable Bio-Factories of Healthy Lipids: Evaluating Fatty Acid Content and Antioxidant Activity.Marine drugs · 2021Article
- Plastidial acyl carrier protein Δ9-desaturase modulates eicosapentaenoic acid biosynthesis and triacylglycerol accumulation in Phaeodactylum tricornutum.The Plant journal : for cell and molecular biology · 2021Article
- Microalgae n-3 PUFAs Production and Use in Food and Feed Industries.Marine drugs · 2021Review
- In Silico Analysis of Partial Fatty Acid Desaturase 2 cDNA FromBioinformatics and biology insights · 2021Article
- Unique fatty acid desaturase capacities uncovered inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Stable isotopes of fatty acids: current and future perspectives for advancing trophic ecology.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Study of Synthesis Pathways of the Essential Polyunsaturated Fatty Acid 20:5n-3 in the DiatomBiomolecules · 2020Article
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Authors and funding
9 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In contrast to 16:3 plants like rapeseed (Brassica napus), which contain alpha-linolenic acid (18:3(Delta9,12,15)) and hexadecatrienoic acid (16:3(Delta7,10,13)) as major polyunsaturated fatty acids in leaves, the silica-less diatom Phaeodactylum tricornutum contains eicosapentaenoic acid (EPA; 20:5(Delta5,8,11,14,17)) and a different isomer of hexadecatrienoic acid (16:3(Delta6,9,12)). In this report, we describe the characterization of two cDNAs having sequence homology to Delta12-fatty acid desaturases from higher plants. These cDNAs were shown to code for a microsomal and a plastidial Delta12-desaturase (PtFAD2 and PtFAD6, respectively) by heterologous expression in yeast (Saccharomyces cerevisiae) and Synechococcus, respectively. Using these systems in the presence of exogenously supplied fatty acids, the substrate specificities of the two desaturases were determined and compared with those of the corresponding rapeseed enzymes (BnFAD2 and BnFAD6). The microsomal desaturases were similarly specific for oleic acid (18:1(Delta9)), suggesting that PtFAD2 is involved in the biosynthesis of EPA. In contrast, the plastidial desaturase from the higher plant and the diatom clearly differed. Although the rapeseed plastidial desaturase showed high activity toward the omega9-fatty acids 18:1(Delta9) and 16:1(Delta7), in line with the fatty acid composition of rapeseed leaves, the enzyme of P. tricornutum was highly specific for 16:1(Delta9). Our results indicate that in contrast to EPA, which is synthesized in the microsomes, the hexadecatrienoic acid isomer found in P. tricornutum (16:3(Delta6,9,12)) is of plastidial origin.
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