ArticlePlant physiology2003
Disruption of apyrases inhibits pollen germination in Arabidopsis.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
62 citing papers in PubMed, 141 citations in OpenAlex.
- Evolution and stress-responsive regulation of the apyrase gene family in Chinese cabbage (Frontiers in plant science · 2026Article
- Apyrase in horticultural crops: insights into growth, stress adaptation and quality regulation.Molecular horticulture · 2025Review
- Extracellular ATP: an emerging multifaceted regulator of plant fitness.Plant biotechnology journal · 2025Review
- Genome-Wide Analysis of Soybean Apyrase Gene Family and Functional Characterization of GmAPY1-4 Responses to Aluminum Stress.International journal of molecular sciences · 2025Article
- Growth regulation by apyrases: Insights from altering their expression level in different organisms.Plant physiology · 2024Review
- Growth-inhibiting effects of the unconventional plant APYRASE 7 of Arabidopsis thaliana influences the LRX/RALF/FER growth regulatory module.PLoS genetics · 2024Article
- cis-Golgi phosphate transporters harboring an EXS domain are essential for plant growth and development.Plant physiology · 2023Article
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- Article
- Extracellular ATP targets Arabidopsis RIBONUCLEASE 1 to suppress mycotoxin stress-induced cell death.The New phytologist · 2022Article
- APYRASE1/2 mediate red light-induced de-etiolation growth in Arabidopsis seedlings.Plant physiology · 2022Article
- Constitutive expression of a pea apyrase, psNTP9, increases seed yield in field-grown soybean.Scientific reports · 2022Article
- A matter of time: Red light regulation of APYRASES shapes seedling growth during de-etiolation.Plant physiology · 2022Article
- P2K1 Receptor, Heterotrimeric Gα Protein and CNGC2/4 Are Involved in Extracellular ATP-Promoted Ion Influx in the Pollen ofPlants (Basel, Switzerland) · 2021Article
- Pollen Germination and Pollen Tube Growth in Gymnosperms.Plants (Basel, Switzerland) · 2021Review
- A Compendium ofFrontiers in plant science · 2021Article
- Rho GTPase ROP1 Interactome Analysis Reveals Novel ROP1-Associated Pathways for Pollen Tube Polar Growth in Arabidopsis.International journal of molecular sciences · 2020Article
- Fertilization in flowering plants: an odyssey of sperm cell delivery.Plant molecular biology · 2020Review
- Article
- Extracellular ATP promoted pollen germination and tube growth of Nicotiana tabacum through promoting KPlant reproduction · 2018Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Arabidopsis, we previously identified two highly similar apyrases, AtAPY1 and AtAPY2. Here, T-DNA knockout (KO) mutations of each gene were isolated in a reverse genetic approach. The single KO mutants lacked a discernible phenotype. The double KO mutants, however, exhibited a complete inhibition of pollen germination, and this correlated with positive beta-glucuronidase staining in the pollen of apyrase promoter:beta-glucuronidase fusion transgenic lines. The vast majority of the pollen grains of these mutants were identical to wild type in size, shape, and nuclear state and were viable as assayed by metabolic activity and plasma membrane integrity. Complementation with either AtAPY1 or AtAPY2 cDNA rescued pollen germination, confirming that the phenotype was apyrase specific. Despite the redundancy of the two apyrases in rescue potential, transmission analyses suggested a greater role for AtAPY2 in male gamete success. The effect of mutant apyrase on the transmission through the female gametophyte was only marginal, and embryo development appeared normal in the absence of apyrases. The male-specific double KO mutation is fully penetrant and shows that apyrases play a crucial role in pollen germination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.