ArticleJournal of virology2003
Human rhinovirus type 2 is internalized by clathrin-mediated endocytosis.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
51 citing papers in PubMed, 96 citations in OpenAlex.
- AP2: an indispensable host factor in virus infection.Journal of virology · 2026Review
- Reframing clathrin-mediated viral entry: evidence standards, context dependence and host-directed vulnerabilities.Frontiers in cellular and infection microbiology · 2026Review
- Determination of Binding and Endocytosis of Rhinovirus by Flow Cytometry.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Endosome rupture enables enteroviruses from the family Picornaviridae to infect cells.Communications biology · 2024Article
- TMEM41B Is an Interferon-Stimulated Gene That Promotes Pseudorabies Virus Replication.Journal of virology · 2023Article
- Pseudorabies Virus Inhibits Expression of Liver X Receptors to Assist Viral Infection.Viruses · 2022Article
- NPC1-regulated dynamic of clathrin-coated pits is essential for viral entry.Science China. Life sciences · 2022Article
- SGIV Induced and Exploited Cellular De Novo Fatty Acid Synthesis for Virus Entry and Replication.Viruses · 2022Article
- The role of lipids in the pathophysiology of coronavirus infections.Osong public health and research perspectives · 2021Article
- Viral Interactions with Adaptor-Protein Complexes: A Ubiquitous Trait among Viral Species.International journal of molecular sciences · 2021Review
- Mechanism of Rhinovirus Immunity and Asthma.Frontiers in immunology · 2021Review
- Cryo-Electron Microscopy Structure of Seneca Valley Virus Procapsid.Journal of virology · 2018Article
- Impairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replication.Scientific reports · 2017Article
- Inhibition of endocytic pathways impacts cytomegalovirus maturation.Scientific reports · 2017Article
- Article
- The Unresolved Role of Interferon-λ inFrontiers in immunology · 2017Review
- Human rhinovirus-induced inflammatory responses are inhibited by phosphatidylserine containing liposomes.Mucosal immunology · 2016Article
- ICAM-1 Binding Rhinoviruses A89 and B14 Uncoat in Different Endosomal Compartments.Journal of virology · 2016Article
- Principles of Virus Uncoating: Cues and the Snooker Ball.Traffic (Copenhagen, Denmark) · 2016Review
- Viral entry pathways: the example of common cold viruses.Wiener medizinische Wochenschrift (1946) · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using several approaches, we investigated the importance of clathrin-mediated endocytosis in the uptake of human rhinovirus serotype 2 (HRV2). By means of confocal immunofluorescence microscopy, we show that K(+) depletion strongly reduces HRV2 internalization. Viral uptake was also substantially reduced by extraction of cholesterol from the plasma membrane with methyl-beta-cyclodextrin, which can inhibit clathrin-mediated endocytosis. In accordance with these data, overexpression of dynamin K44A in HeLa cells prevented HRV2 internalization, as judged by confocal immunofluorescence microscopy, and strongly reduced infection. We also demonstrate that HRV2 bound to the surface of HeLa cells is localized in coated pits but not in caveolae. Finally, transient overexpression of the specific dominant-negative inhibitors of clathrin-mediated endocytosis, the SH3 domain of amphiphysin and the C-terminal domain of AP180, potently inhibited internalization of HRV2. Taken together, these results indicate that HRV2 uses clathrin-mediated endocytosis to infect cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.